Synthesis and properties of chromophores of fluorescent proteins

被引：33

作者：

Ivashkin, P. E. ^{[1
]}

Yampolsky, I. V. ^{[1
]}

Lukyanov, K. A. ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2009年 / 35卷 / 06期

关键词：

fluorescent protein; chromoprotein; chromophores; imidazolone; GFP; UNSATURATED 2,4-DISUBSTITUTED 2-IMIDAZOLIN-5-ONES; PHOTOINDUCED PEPTIDE CLEAVAGE; TO-RED CONVERSION; GREEN FLUORESCENCE; GFP-CHROMOPHORE; INTERNAL-CONVERSION; MOLECULAR-STRUCTURE; ABSORPTION-SPECTRA; MODEL CHROMOPHORES; STRUCTURAL BASIS;

D O I：

10.1134/S1068162009060028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the existing approaches to the synthesis of 5-arylidene-3,5-dihydro-4 H-imidazol-4-ones-model chromophores of fluorescent proteins and their nonnatural analogs. We discuss in detail the chemical (acid-base and redox reactions, cis-trans isomery, etc.) and spectral properties of the chromophores and the influence of substitutes and the environment. The study of synthetic chromophores allows for modeling of the photophysical characteristics of fluorescent proteins.

引用

页码：652 / 669

页数：18

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