A new ratiometric fluorescent probe for sensing lysosomal HOCl based on fluorescence resonance energy transfer strategy

被引：40
作者：
Meng, He [1 ]
Huang, Xiao-Qing [1 ]
Lin, Yu [1 ]
Yang, De-Yuan [1 ]
Lv, Yan-Jing [1 ]
Cao, Xiao-Qun [1 ]
Zhang, Gong-Xiao [1 ]
Dong, Jian [1 ]
Shen, Shi-Li [1 ]
机构：
[1] Shandong First Med Univ & Shandong Acad Med Sci, Sch Chem & Pharmaceut Engn, Tai An 271016, Shandong, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2019年 / 223卷
基金：
中国国家自然科学基金;
关键词：
HOCl; Fluorescent probe; FRET; Ratiometric; Lysosomes; HYPOCHLOROUS ACID; FRET PLATFORM; LIVE CELL; MITOCHONDRIA; WATER; DESIGN; BASAL;
D O I：
10.1016/j.saa.2019.117355
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
In this paper, ratiometric imaging of lysosomal HOCl was realized with a molecular probe (CR-Ly) based on fluorescence resonance energy transfer by using coumarin as the donor and rhodamine as acceptor. CR-Ly showed high sensitivity and fast response to HOCl. Moreover, CR-Ly exhibited excellent selectivity and sensitivity for HOCl over other biologically relevant species. Furthermore, it was successfully utilized to image the endogenous HOCl with low cytotoxity. And CR-Ly was capable of targeting lysosomes and monitoring lysosomal hypochlorous acid changes owing to the presence of the morpholine moiety. We believe that probe CR-Ly would be helpful to further research on the HOC-associated diseases in lysosomes. (C) 2019 Elsevier B.V. All rights reserved.
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页数：6
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