Nuclear transport of human DDB protein induced by ultraviolet light

被引：67

作者：

Liu, W ^{[1
]}

Nichols, AF ^{[1
]}

Graham, JA ^{[1
]}

Dualan, R ^{[1
]}

Abbas, A ^{[1
]}

Linn, S ^{[1
]}

机构：

[1] Univ Calif Berkeley, Div Biochem & Mol Biol, Berkeley, CA 94720 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2000年 / 275卷 / 28期

关键词：

D O I：

10.1074/jbc.M000961200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human damage-specific DNA-binding (DDB) protein can be purified as a heterodimer (p48 and p127) that binds to DNA damaged by ultraviolet light. We report here the effects of UV irradiation on the cellular localization of each DDB subunit as a function of time using green fluorescent fusion proteins in three diploid fibroblast strains: repair-proficient IMR-90 and two repair-deficient xeroderma pigmentosum group E strains (XP95TO and XP3RO). Although p48 remained in the nucleus after UV irradiation, a dynamic nuclear accumulation of p127 from the cytoplasm was found after 24 h. In IMR-90 cells, the nuclear localization of p127 corresponded to the up-regulation of p48 mRNA and protein levels and of DDB activity. XP3RO cells showed delayed but similar kinetics with less transport, whereas XP95TO cells appeared to have different kinetics, suggesting that these cells exhibit different defects in p127 translocation. We propose that p48 might act as the transporter for nuclear entry of p127 but that a third factor might be necessary for efficient transportation.

引用

页码：21429 / 21434

页数：6

共 32 条

[11] CORRECTION OF THE DNA-REPAIR DEFECT IN XERODERMA-PIGMENTOSUM GROUP-E BY INJECTION OF A DNA DAMAGE-BINDING PROTEIN [J].

KEENEY, S ;

EKER, APM ;

BRODY, T ;

VERMEULEN, W ;

BOOTSMA, D ;

HOEIJMAKERS, JHJ ;

LINN, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (09) :4053-4056

[12]

KEENEY S, 1993, J BIOL CHEM, V268, P21293

[13] BIOCHEMICAL HETEROGENEITY IN XERODERMA-PIGMENTOSUM COMPLEMENTATION GROUP-E [J].

KEENEY, S ;

WEIN, H ;

LINN, S .

MUTATION RESEARCH, 1992, 273 (01) :49-56

[14] cul-1 is required for cell cycle exit in C-elegans and identifies a novel gene family [J].

Kipreos, ET ;

Lander, LE ;

Wing, JP ;

He, WW ;

Hedgecock, EM .

CELL, 1996, 85 (06) :829-839

[15] Apolipoprotein B gene regulatory factor-2 (BRF-2) is structurally and immunologically highly related to hepatitis B virus X associated protein-1 (XAP-1) [J].

Krishnamoorthy, RR ;

Lee, TH ;

Butel, JS ;

Das, HK .

BIOCHEMISTRY, 1997, 36 (04) :960-969

[16] HEPATITIS-B VIRUS X-PROTEIN INTERACTS WITH A PROBABLE CELLULAR DNA-REPAIR PROTEIN [J].

LEE, TH ;

ELLEDGE, SJ ;

BUTEL, JS .

JOURNAL OF VIROLOGY, 1995, 69 (02) :1107-1114

[17] Identification of a novel cytoplasmic protein that specifically binds to nuclear localization signal motifs [J].

Li, S ;

Ku, CY ;

Farmer, AK ;

Cong, YS ;

Chen, CF ;

Lee, WH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (11) :6183-6189

[18] The V protein of the paramyxovirus SV5 interacts with damage-specific DNA binding protein [J].

Lin, GY ;

Paterson, RG ;

Richardson, CD ;

Lamb, RA .

VIROLOGY, 1998, 249 (01) :189-200

[19]

Mathias N, 1996, MOL CELL BIOL, V16, P6634

[20] RECONSTITUTION OF HUMAN DNA-REPAIR EXCISION NUCLEASE IN A HIGHLY DEFINED SYSTEM [J].

MU, D ;

PARK, CH ;

MATSUNAGA, T ;

HSU, DS ;

REARDON, JT ;

SANCAR, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (06) :2415-2418

← 1 2 3 4 →