Nuclear transport of human DDB protein induced by ultraviolet light

被引：67

作者：

Liu, W ^{[1
]}

Nichols, AF ^{[1
]}

Graham, JA ^{[1
]}

Dualan, R ^{[1
]}

Abbas, A ^{[1
]}

Linn, S ^{[1
]}

机构：

[1] Univ Calif Berkeley, Div Biochem & Mol Biol, Berkeley, CA 94720 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2000年 / 275卷 / 28期

关键词：

D O I：

10.1074/jbc.M000961200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human damage-specific DNA-binding (DDB) protein can be purified as a heterodimer (p48 and p127) that binds to DNA damaged by ultraviolet light. We report here the effects of UV irradiation on the cellular localization of each DDB subunit as a function of time using green fluorescent fusion proteins in three diploid fibroblast strains: repair-proficient IMR-90 and two repair-deficient xeroderma pigmentosum group E strains (XP95TO and XP3RO). Although p48 remained in the nucleus after UV irradiation, a dynamic nuclear accumulation of p127 from the cytoplasm was found after 24 h. In IMR-90 cells, the nuclear localization of p127 corresponded to the up-regulation of p48 mRNA and protein levels and of DDB activity. XP3RO cells showed delayed but similar kinetics with less transport, whereas XP95TO cells appeared to have different kinetics, suggesting that these cells exhibit different defects in p127 translocation. We propose that p48 might act as the transporter for nuclear entry of p127 but that a third factor might be necessary for efficient transportation.

引用

页码：21429 / 21434

页数：6

共 32 条

[1] MAMMALIAN DNA NUCLEOTIDE EXCISION-REPAIR RECONSTITUTED WITH PURIFIED PROTEIN-COMPONENTS [J].

ABOUSSEKHRA, A ;

BIGGERSTAFF, M ;

SHIVJI, MKK ;

VILPO, JA ;

MONCOLLIN, V ;

PODUST, VN ;

PROTIC, M ;

HUBSCHER, U ;

EGLY, JM ;

WOOD, RD .

CELL, 1995, 80 (06) :859-868

[2] RECOGNITION OF A CYTOSINE BASE LESION BY A HUMAN DAMAGE-SPECIFIC DNA-BINDING PROTEIN [J].

CAREW, JA ;

FELDBERG, RS .

NUCLEIC ACIDS RESEARCH, 1985, 13 (01) :303-315

[3] XERODERMA PIGMENTOSUM GROUP-E CELLS LACK A NUCLEAR FACTOR THAT BINDS TO DAMAGED DNA [J].

CHU, G ;

CHANG, E .

SCIENCE, 1988, 242 (4878) :564-567

[4] SUBSTRATE-SPECIFICITY OF A DAMAGE-SPECIFIC DNA-BINDING PROTEIN FROM HUMAN-CELLS [J].

FELDBERG, RS .

NUCLEIC ACIDS RESEARCH, 1980, 8 (05) :1133-1143

[5]

HART GW, 1989, ANNU REV BIOCHEM, V58, P841

[6] DDB, a putative DNA repair protein, can function as a transcriptional partner of E2F1 [J].

Hayes, S ;

Shiyanov, P ;

Chen, XQ ;

Raychaudhuri, P .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (01) :240-249

[7] A CONSTITUTIVE DAMAGE-SPECIFIC DNA-BINDING PROTEIN IS SYNTHESIZED AT HIGHER LEVELS IN UV-IRRADIATED PRIMATE CELLS [J].

HIRSCHFELD, S ;

LEVINE, AS ;

OZATO, K ;

PROTIC, M .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (05) :2041-2048

[8] Nuclear import of RPA in Xenopus egg extracts requires a novel protein XRIPα but not importin α [J].

Jullien, D ;

Görlich, D ;

Laemmli, UK ;

Adachi, Y .

EMBO JOURNAL, 1999, 18 (15) :4348-4358

[9] UV DAMAGE-SPECIFIC DNA-BINDING PROTEIN IN XERODERMA-PIGMENTOSUM COMPLEMENTATION GROUP-E [J].

KATAOKA, H ;

FUJIWARA, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 175 (03) :1139-1143

[10] Functional complementation of xeroderma pigmentosum complementation group E by replication protein A in an in vitro system [J].

Kazantsev, A ;

Mu, D ;

Nichols, AF ;

Zhao, XD ;

Linn, S ;

Sancar, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (10) :5014-5018

← 1 2 3 4 →