Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer

被引：14

作者：

Zhang, Mengnan ^{[1
,2
,5
]}

Ma, Feng ^{[1
,3
]}

Xie, Ruyi ^{[1
]}

Wu, Yao ^{[1
]}

Wu, Meiyan ^{[1
]}

Zhang, Pei ^{[1
]}

Peng, Ying ^{[1
]}

Zhao, Jinjun ^{[4
]}

Xiong, Jing ^{[1
]}

Li, Aimin ^{[1
]}

Cheng Kequan ^{[2
]}

Zhang, Yali ^{[1
]}

Liu, Side ^{[1
]}

Wang, Jide ^{[1
]}

Chen, Xueqing ^{[2
]}

机构：

[1] Southern Med Univ, Nanfang Hosp, Dept Gastroenterol, Guangdong Prov Key Lab Gastroenterol, Guangzhou 510515, Guangdong, Peoples R China

[2] Guangzhou Med Univ, Dept Gastroenterol, Affiliated Hosp 1, Guangzhou 510120, Guangdong, Peoples R China

[3] Jinan Univ, Dept Gastroenterol, Affiliated Hosp 1, Guangzhou 510630, Guangdong, Peoples R China

[4] Southern Med Univ, Nanfang Hosp, Dept Rheumatism, Guangzhou 510515, Guangdong, Peoples R China

[5] Southern Med Univ, Nanfang Hosp, Dept Huiqiao Bldg, Guangzhou 510515, Guangdong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF ONCOLOGY | 2017年 / 50卷 / 05期

关键词：

Srcin1; colorectal cancer; carcinogenesis; metastasis; invasion; TUMOR-CELL PROPERTIES; COLON-CANCER; SODIUM-BUTYRATE; DOWN-REGULATION; BREAST-CANCER; BETA-CATENIN; EXPRESSION; P140CAP; PROLIFERATION; CARCINOMA;

D O I：

10.3892/ijo.2017.3952

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The adaptor protein Srcin1 is a novel Src-binding protein that regulates Src activation through C-terminal Src kinase (Csk). Srcin1 behaves as a tumour suppressor in breast cancer, but the role of Srcin1 in the development of colorectal cancer (CRC) remains unknown. In the present study, Srcin1 expression in normal tissue was examined by tissue micro array and assessed by immunohistochemistry in 10 patients. In addition, the biological impact of Srcin1 knockdown on CRC cells was investigated in vitro and in vivo. The results showed that Srcin1 was expressed in different types of normal human tissues, whereas its expression was increased in human CRC tissues. Srcin1 expression also correlated with tumour progression. The suppression of Srcin1 induced cell differentiation and G0/G1 cell cycle arrest. Furthermore, Srcin1 increased cell growth as well as the capacity of migration and invasion in CRC cells. Srcin1 induced the activation of the Wnt/beta-catenin signalling pathway. Moreover, Srcin1 suppression sensitized cancer cells to 5-fluorouracil (5-FU)-induced apoptosis in vitro and in vivo. Together, these results demonstrate that Srcin1 contributes to CRC carcinogenesis, invasion and metastasis. These findings provide a rationale for a mechanistic approach to CRC treatment based on the development of Srcin1-targeted therapies.

引用

页码：1555 / 1566

页数：12

共 34 条

[21] High expression of protein phosphatase 4 is associated with the aggressive malignant behavior of colorectal carcinoma [J].