Expression of Trichoderma reesei endo-β-glucanase II in silkworm, Bombyx mori L. by using BmNPV/Bac-to-Bac expression system and its bioactivity assay

被引：8
|
作者：
Zhou, Lie [1 ]
Wu, Xiaofeng [2 ]
Lan, Lipan [2 ]
Liu, Jianxin [1 ]
机构：
[1] Zhejiang Univ, Inst Dairy Sci, Minist Educ, Key Lab Mol Anim Nutr, Hangzhou 310029, Zhejiang, Peoples R China
[2] Zhejiang Univ, Inst Sericulture & Apiculture, Hangzhou 310029, Zhejiang, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 01期
基金：
中国国家自然科学基金;
关键词：
BmNPV/Bac-to-Bac; Expression system; Endoglucanase; Feed additive; Silkworm; Trichoderma reesei; HIGH-LEVEL EXPRESSION; BACULOVIRUS; CELLULASE; LARVAE; ENDOGLUCANASE; YEAST;
D O I：
10.1007/s10529-009-0129-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The silkworm, Bombyx mori, was used to produce recombinant endo-beta-glucanase II (rEGII). The EGII gene (egl2) was cloned from the cellulolytic fungus Trichoderma reesei and inserted into B. mori nucleopolyhedrovirus (BmNPV) genome using BmNPV/Bac-to-Bac expression vector. For expression of rEGII, both the BmN cells and B. mori larvae were infected with the recombinant virus. The putative rEGII yield was about 386 mu g per larva and the enzyme activity of the purified rEGII was approx 352 U/mg of rEGII. The optimal activity of this purified protein was observed at 55A degrees C and pH 4, respectively.
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收藏
页码：67 / 72
页数：6
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