APPLICATION OF NEW METHODS FOR DETECTION OF DNA DAMAGE AND REPAIR

被引：3

作者：

Svetlova, Maria R. ^{[1
]}

Solovjeva, Liudmila V. ^{[1
]}

Tomilin, Nikolai V. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Cytol, St Petersburg 194064, Russia

来源：

INTERNATIONAL REVIEW OF CELL AND MOLECULAR BIOLOGY, VOL 277 | 2009年 / 277卷

关键词：

DNA repair; DNA damage; Chromatin modifications; Genome damage; Detection methods; NUCLEOTIDE EXCISION-REPAIR; CELL NUCLEAR ANTIGEN; DOUBLE-STRAND BREAKS; PHOSPHORYLATED HISTONE H2AX; PCNA COMPLEX-FORMATION; TRANSCRIPTION-COUPLED REPAIR; GROUP-A PROTEIN; XERODERMA-PIGMENTOSUM; S-PHASE; UV-IRRADIATION;

D O I：

10.1016/S1937-6448(09)77006-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

New methods for detecting DNA damage and repair are reviewed and their potential significance is discussed. These include methods based on analysis of DNA damage-induced chromatin modifications, cytological detection of DNA repair synthesis, damage-induced immobilization of repair proteins and living cell imaging. Special attention is paid to current methods of detection of modifications of histories and other proteins associated with DNA double-strand breaks which represent most dangerous genome damage. New methods of analysis of DNA damage and repair may be useful in biodosimetry, early cancer diagnostics and in the analysis of efficiency of cancer radiation therapy and chemotherapy.

引用

页码：217 / 251

页数：35

共 179 条

[1] DETECTION OF NUCLEOTIDE EXCISION-REPAIR INCISIONS IN HUMAN FIBROBLASTS BY IMMUNOSTAINING FOR PCNA [J].

ABOUSSEKHRA, A ;

WOOD, RD .

EXPERIMENTAL CELL RESEARCH, 1995, 221 (02) :326-332

[2] DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation [J].

Bakkenist, CJ ;

Kastan, MB .

NATURE, 2003, 421 (6922) :499-506

[3] Efficient PCNA complex formation is dependent upon both transcription coupled repair and genome overall repair [J].

Balajee, AS ;

May, A ;

Dianova, I ;

Bohr, VA .

MUTATION RESEARCH-DNA REPAIR, 1998, 409 (03) :135-146

[4] Radiation sensitivity, H2AX phosphorylation, and kinetics of repair of DNA strand breaks in irradiated cervical cancer cell lines [J].

Banáth, JP ;

MacPhail, SH ;

Olive, PL .

CANCER RESEARCH, 2004, 64 (19) :7144-7149

[5]

BARBAT JG, 2008, MOL BIOL CELL, V19, P3969

[6] Repair and genetic consequences of endogenous DNA base damage in mammalian cells [J].

Barnes, DE ;

Lindahl, T .

ANNUAL REVIEW OF GENETICS, 2004, 38 :445-476

[7] DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis [J].

Bartkova, J ;

Horejsi, Z ;

Koed, K ;

Krämer, A ;

Tort, F ;

Zieger, K ;

Guldberg, P ;

Sehested, M ;

Nesland, JM ;

Lukas, C ;

Orntoft, T ;

Lukas, J ;

Bartek, J .

NATURE, 2005, 434 (7035) :864-870

[8] Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks [J].

Bekker-Jensen, S ;

Lukas, C ;

Kitagawa, R ;

Melander, F ;

Kastan, MB ;

Bartek, J ;

Lukas, J .

JOURNAL OF CELL BIOLOGY, 2006, 173 (02) :195-206

[9] DNA damage triggers nucleotide excision repair-dependent monoubiquitylation of histone H2A [J].

Bergink, Steven ;

Salomons, Florian A. ;

Hoogstraten, Deborah ;

Groothuis, Tom A. M. ;

de Waard, Harm ;

Wu, Junxin ;

Yuan, Li ;

Citterio, Elisabetta ;

Houtsmuller, Adriaan B. ;

Neefjes, Jacques ;

Hoeijmakers, Ian H. J. ;

Vermeulen, Wim ;

Dantuma, Nico P. .

GENES & DEVELOPMENT, 2006, 20 (10) :1343-1352

[10] Regulation of UV-induced DNA damage response by ubiquitylation [J].

Beryink, Steuen ;

Jaspers, Nicolaas G. J. ;

Vermeulen, Wim .

DNA REPAIR, 2007, 6 (09) :1231-1242

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