Raman microspectroscopy, surface-enhanced Raman scattering microspectroscopy, and stable-isotope Raman microspectroscopy for biofilm characterization

被引:67
作者
Ivleva, Natalia P. [1 ]
Kubryk, Patrick [1 ]
Niessner, Reinhard [1 ]
机构
[1] Tech Univ Munich, Inst Hydrochem, Chair Analyt Chem, Marchioninistr 173, D-81377 Munich, Germany
关键词
Biofilm; Microorganisms; Extracellular polymeric substances; Biofilm characterization; Raman microspectroscopy; Resonance Raman microspectroscopy; Surface-enhanced Raman scattering; Stable-isotope Raman microspectroscopy; IN-SITU HYBRIDIZATION; SINGLE-MOLECULE SERS; MICROBIAL BIOFILMS; BACTERIAL BIOFILMS; MASS-SPECTROMETRY; SPECTROSCOPIC IDENTIFICATION; RAPID IDENTIFICATION; MICROSCOPIC ANALYSIS; CHEMICAL-COMPOSITION; CELL ANALYSIS;
D O I
10.1007/s00216-017-0303-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Biofilms represent the predominant form of microbial life on our planet. These aggregates of microorganisms, which are embedded in a matrix formed by extracellular polymeric substances, may colonize nearly all interfaces. Detailed knowledge of microorganisms enclosed in biofilms as well as of the chemical composition, structure, and functions of the complex biofilm matrix and their changes at different stages of the biofilm formation and under various physical and chemical conditions is relevant in different fields. Important research topics include the development and improvement of antibiotics and medical devices and the optimization of biocides, antifouling strategies, and biological wastewater treatment. Raman microspectroscopy is a capable and nondestructive tool that can provide detailed two-dimensional and three-dimensional chemical information about biofilm constituents with the spatial resolution of an optical microscope and without interference from water. However, the sensitivity of Raman microspectroscopy is rather limited, which hampers the applicability of Raman microspectroscopy especially at low biomass concentrations. Fortunately, the resonance Raman effect as well as surface-enhanced Raman scattering can help to overcome this drawback. Furthermore, the combination of Raman microspectroscopy with other microscopic techniques, mass spectrometry techniques, or particularly with stable-isotope techniques can provide comprehensive information on monospecies and multispecies biofilms. Here, an overview of different Raman microspectroscopic techniques, including resonance Raman microspectroscopy and surface-enhanced Raman scattering microspectroscopy, for in situ detection, visualization, identification, and chemical characterization of biofilms is given, and the main feasibilities and limitations of these techniques in biofilm research are presented. Future possibilities of and challenges for Raman microspectroscopy alone and in combination with other analytical techniques for characterization of complex biofilm matrices are discussed in a critical review.
引用
收藏
页码:4353 / 4375
页数:23
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