The complete genome sequence of Bacillus velezensis 9912D reveals its biocontrol mechanism as a novel commercial biological fungicide agent

被引：33
作者：
Pan, Hua-Qi [1 ]
Li, Qing-Lian [2 ]
Hu, Jiang-Chun [1 ]
机构：
[1] Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
[2] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
来源：
JOURNAL OF BIOTECHNOLOGY | 2017年 / 247卷
基金：
中国国家自然科学基金;
关键词：
Bacillus velezensis; Bacillus methylotrophicus; Genome; Biocontrol; Secondary metabolite; LATER HETEROTYPIC SYNONYM; AMYLOLIQUEFACIENS FZB42; ANALYSIS SHOWS; SURFACTIN; GROWTH;
D O I：
10.1016/j.jbiotec.2017.02.022
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Bacillus sp. 9912 mutant, 9912D, was approved as a new biological fungicide agent by the Ministry of Agriculture of the People's Republic of China in 2016 owing to its excellent inhibitory effect on various plant pathogens and being environment-friendly. Here, we present the genome of 9912D with a circular chromosome having 4436 coding DNA sequences (CDSs), and a circular plasmid encoding 59 CDSs. This strain was finally designated as Bacillus velezensis based on phylogenomic analyses. Genome analysis revealed a total of 19 candidate gene clusters involved in secondary metabolite biosynthesis, including potential new type II antibiotics. The absence of fengycin biosynthetic gene cluster is noteworthy. Our data offer insights into the genetic, biological and physiological characteristics of this strain and aid in deeper understanding of its biocontrol mechanism.(C) 2017 Elsevier B.V. All rights reserved.
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页码：25 / 28
页数：4
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