Copper improves the production of laccase by the white-rot fungus Pleurotus pulmonarius in solid state fermentation

被引:50
作者
Tychanowicz, Giovana Kirst
de Souza, Daniela F.
Souza, Cristina G. M.
Kadowaki, Marina Kirniko
Peralta, Rosane Marina [1 ]
机构
[1] Univ Estadual Maringa, Dept Bioquim, Maringa, Parana, Brazil
[2] Univ Estadual Maringa, Dept Agron, Maringa, Parana, Brazil
[3] Univ Estasual Cascavel, Cascavel, PR, Brazil
关键词
copper; laccase; Pleurotus pulmonarius; phenoloxidase; solid state fermentation;
D O I
10.1590/S1516-89132006000600002
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Pleurotus pulmonarius (Fr) Quelet, produced laccase as the main ligninolytic enzyme when cultivated on solid-state cultures using corn cob as substrate. The addition of copper greatly increased the production of enzyme. The addition of 25.0 mM CuSO4 increased the level of laccase from 270 to 1,420 U.L-1 and the fungus showed high resistance to copper under the conditions used in this work.
引用
收藏
页码:699 / 704
页数:6
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