RAPD and ISSR molecular markers in Olea europaea L.:: Genetic variability and molecular cultivar identification

被引:66
作者
Martins-Lopes, Paula
Lima-Brito, Jose
Gomes, Sonia
Meirinhos, Julieta
Santos, Luis
Guedes-Pinto, Henrique
机构
[1] Univ Tras Os Montes & Alto Douro, Dept Genet & Biotechnol, Ctr Genet & Biotechnol, P-5000911 Vila Real, Portugal
[2] Natl Plant Breeding Stn, Dept Oliviculture, P-7350951 Elvas, Portugal
关键词
genetic variability; ISSR; molecular cultivar identification; Olea europaea L; RAPD;
D O I
10.1007/s10722-005-2640-7
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Thirty Portuguese and eight foreign olive (Olea europaea L.) cultivars were screened using Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) markers. Twenty RAPD primers amplified 301 reproducible bands of which 262 were polymorphic; and 17 ISSR primers amplified 204 bands of which 180 were polymorphic. The percentage of polymorphic bands detected by ISSR and RAPD was similar (88 and 87%, respectively). The genetic variability observed was similar in the Portuguese and foreign olive cultivars. Seven ISSR and 12 RAPD primers were able to distinguish individually all 38 olive cultivars. Twenty specific molecular markers are now available to be converted into Sequence Characterised Amplified Region (SCAR) markers. Relationships among Portuguese and foreign cultivars is discussed.
引用
收藏
页码:117 / 128
页数:12
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