Biological Nanopore Approach for Single-Molecule Protein Sequencing

被引:169
|
作者
Hu, Zheng-Li [1 ]
Huo, Ming-Zhu [1 ]
Ying, Yi-Lun [1 ,2 ]
Long, Yi-Tao [1 ]
机构
[1] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, 163 Xianlin Ave, Nanjing 210023, Peoples R China
[2] Nanjing Univ, Chem & Biomed Innovat Ctr, 163 Xianlin Ave, Nanjing 210023, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
biological nanopore; diagnostics; nano-confinement; protein sequencing; single-molecule sensing;
D O I
10.1002/anie.202013462
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Proteins are responsible for the occurrence and treatment of many diseases, and therefore protein sequencing will revolutionize proteomics and clinical diagnostics. Biological nanopore approach has proved successful for single-molecule DNA sequencing, which resolves the identities of 4 natural deoxyribonucleotides based on the current blockages and duration times of their translocations across the nanopore confinement. However, open challenges still remain for biological nanopores to sequentially identify each amino acid (AA) of single proteins due to the inherent complexity of 20 proteinogenic AAs in charges, volumes, hydrophobicity and structures. Herein, we focus on recent exciting advances in biological nanopores for single-molecule protein sequencing (SMPS) from native protein unfolding, control of peptide translocation, AA identification to applications in disease detection.
引用
收藏
页码:14738 / 14749
页数:12
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