Comparison between optical coherence tomography imaging and histological sections of peripheral nerves

被引:5
作者
Carolus, Anne E. [1 ]
Moeller, Jens [2 ]
Hofmann, Martin R. [2 ]
van de Nes, Johannes A. P. [3 ]
Welp, Hubert [4 ]
Schmieder, Kirsten [1 ]
Brenke, Christopher [1 ]
机构
[1] Ruhr Univ Bochum, Univ Hosp Knappschaftskrankenhaus Bochum, Dept Neurosurg, Bochum, Germany
[2] Ruhr Univ Bochum, Dept Photon & Terahertz Technol, Bochum, Germany
[3] Ruhr Univ Bochum, Univ Hosp Bergmannsheil Bochum, Dept Pathol, Bochum, Germany
[4] Tech Hsch Georg Agr, Bochum, Germany
关键词
optical coherence tomography; image-processing technique; histological section; histological staining; myelin; axon; interfascicular tissue; peripheral nerve; diagnostic technique;
D O I
10.3171/2019.8.JNS191278
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
OBJECTIVE Optical coherence tomography (OCT) is an imaging technique that uses the light-backscattering properties of different tissue types to generate an image. In an earlier feasibility study the authors showed that it can be applied to visualize human peripheral nerves. As a follow-up, this paper focuses on the interpretation of the images obtained. METHODS Ten different short peripheral nerve specimens were retained following surgery. In a first step they were examined by OCT during, or directly after, surgery. In a second step the nerve specimens were subjected to histological examination. Various steps of image processing were applied to the OCT raw data acquired. The improved OCT images were compared with the sections stained by H & E. The authors assigned the structures in the images to the various nerve components including perineurium, fascicles, and intrafascicular microstructures. RESULTS The results show that OCT is able to resolve the myelinated axons. A weighted averaging filter helps in identifying the borders of structural features and reduces artifacts at the same time. Tissue-remodeling processes due to injury (perineural fibrosis or neuroma) led to more homogeneous light backscattering. Anterograde axonal degeneration due to sharp injury led to a loss of visible axons and to an increase of light-backscattering tissue as well. However, the depth of light penetration is too small to allow generation of a complete picture of the nerve. CONCLUSIONS OCT is the first in vivo imaging technique that is able to resolve a nerve's structures down to the level of myelinated axons. It can yield information about focal and segmental pathologies.
引用
收藏
页码:270 / 277
页数:8
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