The solution structure of the PufX polypeptide from Rhodobacter sphaeroides

被引：31
作者：
Tunnicliffe, Richard B. [1 ]
Ratcliffe, Emma C. [1 ]
Hunter, C. Neil [1 ]
Williamson, Mike P. [1 ]
机构：
[1] Univ Sheffield, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England
来源：
FEBS LETTERS | 2006年 / 580卷 / 30期
基金：
英国生物技术与生命科学研究理事会;
关键词：
photosynthesis; reaction centre; NMR structure; light harvesting; membrane protein; Rhodobacter sphaeroides;
D O I：
10.1016/j.febslet.2006.11.065
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
PufX organises the photosynthetic reaction centrelight harvesting complex 1 (RC-LH1) core complex of Rhodobacter sphaeroides and facilitates quinol/quinone exchange between the RC and cytochrome bc(1) complexes. The structure of PufX in organic solvent reveals two hydrophobic helices flanked by unstructured termini and connected by a helical bend. The proposed location of basic residues and tryptophans at the membrane interface orients the C-terminal helix along the membrane normal, with the GXXXG motifs in positions unsuitable as direct drivers of dimerisation of the RC-LH1 complex. The Nterminal helix is predicted to extend similar to 40 angstrom along the membrane interface. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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页码：6967 / 6971
页数：5
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