In vivo brain imaging using a portable 2.9 g two-photon microscope based on a microelectromechanical systems scanning mirror

被引：138

作者：

Piyawattanametha, Wibool ^{[1
,2
]}

Cocker, Eric D. ^{[1
]}

Burns, Laurie D. ^{[1
]}

Barretto, Robert P. J. ^{[1
]}

Jung, Juergen C. ^{[1
]}

Ra, Hyejun ^{[3
]}

Solgaard, Olav ^{[3
]}

Schnitzer, Mark J. ^{[1
,4
]}

机构：

[1] Stanford Univ, James H Clark Ctr Biomed Engn & Sci, Stanford, CA 94305 USA

[2] Natl Elect & Comp Technol Ctr, Pathum Thani 12120, Thailand

[3] Stanford Univ, Edward L Ginzton Lab, Stanford, CA 94305 USA

[4] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA

来源：

OPTICS LETTERS | 2009年 / 34卷 / 15期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

PHOTONIC CRYSTAL FIBER; FLUORESCENCE MICROENDOSCOPE; MINIATURIZED PROBE; HIGH-SPEED; ENDOSCOPY; LASER; MICE; LENS;

D O I：

10.1364/OL.34.002309

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present a two-photon microscope that is approximately 2.9 g in mass and 2.0 x 1.9 x 1.1 cm(3) in size and based on a microelectromechanical systems (MEMS) laser-scanning mirror. The microscope has a focusing motor and a micro-optical assembly composed of four gradient refractive index lenses and a dichroic microprism. Fluorescence is captured without the detected emissions reflecting off the MEMS mirror, by use of separate optical fibers for fluorescence collection and delivery of ultrashort excitation pulses. Using this microscope we imaged neocortical microvasculature and tracked the flow of erythrocytes in live mice. (C) 2009 Optical Society of America

引用

页码：2309 / 2311

页数：3

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