Isolation, purification and characterization of antioxidative peptide of pearl millet (Pennisetum glaucum) protein hydrolysate

被引:180
作者
Agrawal, Himani [1 ]
Joshi, Robin [1 ]
Gupta, Mahesh [1 ]
机构
[1] CSIR Inst Himalayan Bioresource, Palampur, Himachal Prades, India
关键词
Pearl millet; Antioxidant; Peptide purification; MALDI-TOF-MS; RP-UFLC; DIFFERENTIAL SCANNING CALORIMETRY; ENZYMATIC HYDROLYSATE; BIOACTIVE PEPTIDES; IDENTIFICATION; CAPACITY; QUALITY;
D O I
10.1016/j.foodchem.2016.02.127
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Pearl millet (Pennisetum glaucum) is a rich source of protein, used for present study to hydrolyze protein, peptide separation and its functional activity. Antioxidative bioactive peptide was successfully identified from pearl millet using trypsin enzyme. Different antioxidative potential of isolated peptide were assessed based on activity of DPPH radical, ABTS radical, hydroxyl radical, Fe2+ chelating ability and reducing power. Bioactive peptide separated by gel-filtration chromatography, showed the higher antioxidant activity as tested by different free radicals. The activity of pearl millet protein hydrolysate fraction was found for DPPH assay (67.66%), ABTS assay (78.81%), Fe2+ chelating ability (51.20%), hydroxyl assay (60.95%) and reducing power (0.375 nm) was further purified using reversed-phase UFLC and subjected to matrix assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDITOF/TOF-MS) for sequential identification of the peptide. The sequence SDRDLLGPNNQYLPK was identified as antioxidant peptide. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:365 / 372
页数:8
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