Background: Enteric nervous system abnormalities have been described in patients with inflammatory bowel diseases. However, the mechanisms responsible for these abnormalities remain to date Largely unknown. Aims: We investigated the potential rote of apoptotic phenomena in enteric neurons and enteroglial cells in patients with inflammatory bowel diseases. Patients and methods: Full-thickness surgical specimens of 19 patients undergoing surgery for medically refractory disease (9 from the ileum of patients with Crohn's disease, 10 from the colon of patients with ulcerative colitis) were assessed for the presence of enteric neurons and enteroglial cells and for their apoptosis by two immunohistochemical methods, one also able to distinguish apoptosis from necrosis. The results were compared with those obtained in control specimens. Results: Concerning Crohn's disease, the ileal segments displayed a significant increase of apoptotic enteric neurons and enteroglial. cells in both the submucous and the myenteric plexus compared to controls. In patients with ulcerative colitis, compared to controls, apoptotic phenomena were significantly reduced in enteric neurons, whereas they were increased in the enteroglial cell population (submucous and myenteric plexus). Conclusions: In patients with inflammatory bowel disease apoptotic phenomena involve both enteric neurons and enteroglial cells, and may play a rote in the abnormalities of the enteric nervous system. The importance of these findings in the pathophysiology of these conditions remains to be determined. (C) 2009 European Crohn's and Colitis Organisation. Published by Elsevier B.V. All rights reserved.