Purification and characterization of a lectin from endophytic fungus Fusarium solani having complex sugar specificity

被引：53

作者：

Khan, Feroz ^{[1
]}

Ahmad, Absar ^{[1
]}

Khan, M. Islam ^{[1
]}

机构：

[1] Natl Chem Lab, Div Biochem Sci, Pune 411008, Maharashtra, India

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2007年 / 457卷 / 02期

关键词：

endophytic fungus; Fusarium solani; lectin; purification; thermodynamic properties; SPR;

D O I：

10.1016/j.abb.2006.10.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A lectin from the mycelial extract of an endophytic strain of Fusarium solani was purified. Its hemagglutinating activity was inhibited by glycoproteins possessing N-linked as well as O-linked glycans. The thermodynamics and kinetics of binding of glycans and glycoproteins to F. solani lectin Was Studied using SUH ace plasmon resonance. The lectin showed high affinity for asialofetuin, asia-lofibrinogen, asialofibrinogen, and thyroglobulin: and comparatively low affinity for mucin, fetuin, fibrinogen, and holotransferrin. Glycoproteins showed glycans with significant contribution from enthalpy and positive entropy, suggesting several fold higher affinity than their corresponding g the involvement of non-polar protein-protein interaction. Moreover, the higher affinity of the glycoproteins was due to their faster association rates and low activation energy. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：243 / 251

页数：9

共 36 条

[31] PURIFICATION AND PROPERTIES OF AN N-ACETYLGALACTOSAMINE SPECIFIC LECTIN FROM THE PLANT PATHOGENIC FUNGUS RHIZOCTONIA-SOLANI [J].

VRANKEN, AM ;

VANDAMME, EJM ;

ALLEN, AK ;

PEUMANS, WJ .

FEBS LETTERS, 1987, 216 (01) :67-72

[32] Lectins from mushrooms [J].

Wang, HX ;

Ng, TB ;

Ooi, VEC .

MYCOLOGICAL RESEARCH, 1998, 102 :897-906

[33] A new lectin with highly potent antihepatoma and antisarcoma activities from the oyster mushroom Pleurotus ostreatus [J].

Wang, HX ;

Gao, JQ ;

Ng, TB .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 275 (03) :810-816

[34]

Wasser SP, 1999, CRIT REV IMMUNOL, V19, P65

[35]

WEBER K, 1969, J BIOL CHEM, V244, P4406

[36] Carbohydrate specificity of a lectin isolated from the fungus Sclerotium rolfsii [J].

Wu, AM ;

Wu, JH ;

Tsai, MS ;

Hegde, GV ;

Inamdar, SR ;

Swamy, BM ;

Herp, A .

LIFE SCIENCES, 2001, 69 (17) :2039-2050

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