Are Monocyte Chemoattractant Protein-1-2518 G and Regulated Activation Normal T Cell Expressed and Secreted-403 A Alleles Risk Factor for Periodontitis?

Objective: We aimed to analyze the monocyte chemoattractant protein-1 (MCP-1) and regulated on activation, normal T cell expressed and secreted (RANTES) polymorphisms in relation to susceptibility to chronic periodontitis and influence of genotypes in response to non-surgical periodontal therapy. Methods: This cross-sectional clinical study was performed during 2005-2006 in Turkey. Gene polymorphisms were investigated in 51 periodontitis patients and 48 periodontally healthy subjects. Quadrant scaling and root planning was performed to periodontitis patients. Genomic DNA was analyzed for polymorphisms by polymerase chain reaction amplification followed by restriction enzyme digestion. Results: Genotype distributions of related genes were similar between periodontitis and healthy subjects (P>0.05). In periodontitis group, monocyte chemoattractant protein-1 -2518G allele frequencies were 25.5%, regulated on activation, normal T cell expressed and secreted-403A allele frequencies were 30.4%. Percentage of bleeding sites were significantly higher in regulated on activation, normal T cell expressed and secreted gene A negative genotypes compared to A positive (P=0.023). Significant improvements in clinical parameters were found after periodontal therapy in both genotypes (P<0.05). Conclusion: Within the limits of present data, results may suggest that there were no association between specific alleles and periodontal treatment effect. Furthermore, these gene polymorphisms are not associated with chronic periodontitis in Turkish population.