Effects of α-Melanocortin Enantiomers on Acetaminophen-Induced Hepatotoxicity in CBA Mice

被引：10

作者：

Turcic, Petra ^{[2
]}

Bradamante, Mirna ^{[3
]}

Houra, Karlo ^{[4
]}

Stambuk, Nikola ^{[1
]}

Kelava, Tomislav ^{[5
]}

Konjevoda, Pasko ^{[1
]}

Kazazic, Sasa ^{[1
]}

Vikic-Topic, Drazen ^{[1
]}

Pokric, Biserka ^{[1
]}

机构：

[1] Rudjer Boskovic Inst, Zagreb 10002, Croatia

[2] Univ Zagreb, Dept Pharmacol, Fac Pharm & Biochem, Zagreb 10000, Croatia

[3] Univ Hosp, Ctr Zagreb, Dept Dermatol & Venerol, Zagreb 10000, Croatia

[4] Univ Hosp Sestre Milosrdnice, Dept Neurosurg, Zagreb 10000, Croatia

[5] Univ Zagreb, Sch Med, Dept Physiol & Immunol, Zagreb 10000, Croatia

来源：

MOLECULES | 2009年 / 14卷 / 12期

关键词：

enantiomer; alpha-MSH; hepatoprotection; hepatotoxicity; antibody; CD spectroscopy; D-AMINO ACIDS; MELANOCYTE-STIMULATING HORMONE; IN-VITRO; PEPTIDES;

D O I：

10.3390/molecules14125017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins and peptides in mammals are based exclusively on L-amino acids. Recent investigations show that D-amino acids exhibit physiological effects in vivo, despite of their very small quantities. We have investigated the hepatoprotective effects of the Land D-enantiomers of alpha-melanocortin peptide (alpha-MSH). The results showed that peptide-enantiomerism is related to the protective effects of melanocortin peptides in vivo. L-alpha-MSH exhibited potent hepatoprotective effect in the experimental model of acetaminophen induced hepatotoxicity in male CBA mice, while its D-mirror image was inefficient. Furthermore, the antibody to the L-peptide did not recognize the D-structure. The results indicate that the opposite peptide configuration may be used to modulate its function and metabolism in vivo and in vitro.

引用

页码：5017 / 5026

页数：10

共 29 条

[1] CROSS-REACTIVITY OF ANTIBODIES TO RETRO-INVERSO PEPTIDOMIMETICS WITH THE PARENT PROTEIN HISTONE H3 AND CHROMATIN CORE PARTICLE - SPECIFICITY AND KINETIC RATE-CONSTANT MEASUREMENTS [J].