Overexpression of Plk1 promotes malignant progress in human esophageal squamous cell carcinoma

被引:29
作者
Zhao, Chunling [1 ]
Gong, Lei [2 ]
Li, Wentong [1 ]
Chen, Limei [1 ]
机构
[1] Weifang Med Univ, Basic Med Dept, Weifang 261053, Shandong, Peoples R China
[2] Shandong Univ, Sch Med, Jinan, Shandong, Peoples R China
关键词
Plk1; Overexpression; ESCC; Small interfering RNA; Proliferation; Migration; POLO-LIKE-KINASE; CANCER-CELLS; PROGNOSTIC-SIGNIFICANCE; LUNG-CANCER; CYCLIN B1; EXPRESSION; APOPTOSIS; INHIBITION; ACTIVATION; GROWTH;
D O I
10.1007/s00432-009-0630-4
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Plk1, belonging to a family of serine/threonine kinases, is involved in spindle formation and chromosome segregation during mitosis and therefore, in the regulation of cell cycle. Plk1 was found to be overexpressed in various human tumors. In the present work, we investigated the expression of human esophageal squamous cell carcinoma (ESCC) to determine whether Plk1 has a role in malignant progress. Immunohistochemistry and Western blotting were performed to define the expressions of Plk1 in ESCC tissues and normal adjacent tissues. Transfection of cells with small interference RNA, growth suppression assay, and Transwell assay were used to determine the potential role of Plk1 in ESCC malignant progress. Plk1 was overexpressed in 69.6% of the ESCC tissues. In addition, the extent of Plk1 expression was closely correlated with differentiation grades and invasiveness in ESCC. We also found that the downregulation of endogenous Plk1 in human ESSC cell lines Eca-109 and EC9706 significantly decreased cells proliferation and migrating ability. Our results show that Plk1 expression is elevated in ESCC tissues and is associated with differentiation grades and invasiveness in ESCC, indicating that overexpression of Plk1 may play an important role in carcinogenesis and malignant progress of ESCC.
引用
收藏
页码:9 / 16
页数:8
相关论文
共 29 条
[1]   Polo-like kinase (Plk) 1: a novel target for the treatment of prostate cancer [J].
Ahmad, N .
FASEB JOURNAL, 2004, 18 (01) :5-7
[2]   Silencing of polo-like kinase (Plk) 1 via siRNA causes inhibition of growth and induction of apoptosis in human esophageal cancer cells [J].
Bu, Youquan ;
Yang, Zhengmei ;
Li, Quanhai ;
Song, Fangzhou .
ONCOLOGY, 2008, 74 (3-4) :198-206
[3]   Overexpression of PLK1 is associated with poor survival by inhibiting apoptosis via enhancement of survivin level in esophageal squamous cell carcinoma [J].
Feng, Yan-Bin ;
Lin, De-Chen ;
Shi, Zhi-Zhou ;
Wang, Xiao-Chun ;
Shen, Xiao-Ming ;
Zhang, Yu ;
Du, Xiao-Li ;
Luo, Man-Li ;
Xu, Xin ;
Han, Ya-Ling ;
Cai, Yan ;
Zhang, Zi-Qiang ;
Zhan, Qi-Min ;
Wang, Ming-Rong .
INTERNATIONAL JOURNAL OF CANCER, 2009, 124 (03) :578-588
[4]   Polo-like kinases: a team that plays throughout mitosis [J].
Glover, DM ;
Hagan, IM ;
Tavares, AAM .
GENES & DEVELOPMENT, 1998, 12 (24) :3777-3787
[5]   Administration of PLK-1 small interfering RNA with atelocollagen prevents the growth of liver metastases of lung cancer [J].
Kawata, Eri ;
Ashihara, Eishi ;
Kimura, Shinya ;
Takenaka, Kazumasa ;
Sato, Kiyoshi ;
Tanaka, Ruriko ;
Yokota, Asumi ;
Kamitsuji, Yuri ;
Takeuchi, Miki ;
Kuroda, Junya ;
Tanaka, Fumihiro ;
Yoshikawa, Toshikazu ;
Maekawa, Taira .
MOLECULAR CANCER THERAPEUTICS, 2008, 7 (09) :2904-2912
[6]  
Knecht R, 1999, CANCER RES, V59, P2794
[7]  
Knecht R, 2000, INT J CANCER, V89, P535
[8]   Activation of Cdc2/cyclin B and inhibition of centrosome amplification in cells depleted of Plk1 by siRNA [J].
Liu, XQ ;
Erikson, RL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (13) :8672-8676
[9]   Polo-like kinase (Plk)1 depletion induces apoptosis in cancer cells [J].
Liu, XQ ;
Erikson, RL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (10) :5789-5794
[10]   Genetic steps in the development of squamous cell carcinoma of the esophagus [J].
Mandard, AM ;
Hainaut, P ;
Hollstein, M .
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2000, 462 (2-3) :335-342