Transmission of Porcine Circovirus 3 (PCV3) by Xenotransplantation of Pig Hearts into Baboons

被引：46

作者：

Krueger, Luise ^{[1
]}

Laengin, Matthias ^{[2
]}

Reichart, Bruno ^{[3
]}

Fiebig, Uwe ^{[1
]}

Kristiansen, Yannick ^{[1
]}

Prinz, Carolin ^{[1
]}

Kessler, Barbara ^{[4
]}

Egerer, Stefanie ^{[4
]}

Wolf, Eckhard ^{[4
]}

Abicht, Jan-Michael ^{[2
]}

Denner, Joachim ^{[5
]}

机构：

[1] Robert Koch Inst, HIV & Other Retroviruses, D-13353 Berlin, Germany

[2] Ludwig Maximilians Univ Munchen, Dept Anaesthesiol, D-81377 Munich, Germany

[3] Ludwig Maximilians Univ Munchen, Walter Brendel Ctr Expt Med, D-81377 Munich, Germany

[4] Ludwig Maximilians Univ Munchen, Gene Ctr, Mol Anim Breeding & Biotechnol, D-85764 Oberschleissheim, Germany

[5] Robert Koch Inst, D-13353 Berlin, Germany

来源：

VIRUSES-BASEL | 2019年 / 11卷 / 07期

关键词：

porcine circovirus 3 (PCV3); pigs; non-human primates; xenotransplantation; ISLET-CELL TRANSPLANTATION; MICROBIOLOGICAL CHARACTERIZATION; GOTTINGEN MINIPIGS; DISEASE; CYTOMEGALOVIRUS; PATHOGENESIS; REPLICATION; INFECTION; PCR;

D O I：

10.3390/v11070650

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Porcine circovirus 3 (PCV3) is a newly described member of the virus family Circoviridae. PCV3 is highly distributed among pigs and wild boars worldwide. A sudden introduction of PCV3 was recently observed in a herd of triple genetically modified pigs generated for xenotransplantation. These animals were used as donor pigs for orthotopic heart transplantation into baboons. In four cases, PCV3-positive hearts were transplanted, and transmission of PCV3 to the recipient was observed. PCV3 was found in all organs of the recipient baboons and a higher virus load was found in animals with a longer survival time of the transplant, indicating replication of the virus. This is the first report showing trans-species transmission of PCV3 to baboons by transplantation of a heart from a PCV3-positive donor pig. Sequence analysis showed that PCV3a and PCV3b were present in the infected pigs and were transmitted. Experiments to infect human 293 cells with PCV3 failed.

引用

页数：11

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