A bacterial glycosyltransferase gene toolbox: Generation and applications

被引：34
|
作者：
Erb, Annette [1 ]
Weiss, Holger [1 ]
Haerle, Johannes [1 ]
Bechthold, Andreas [1 ]
机构：
[1] Univ Freiburg, Inst Pharmazeut Wissensch Pharmazeut Biol & Biote, D-79104 Freiburg, Germany
来源：
PHYTOCHEMISTRY | 2009年 / 70卷 / 15-16期
关键词：
Streptomyces; Combinatorial biosynthesis; Glycosyltransferase; NATURAL-PRODUCT GLYCOSYLTRANSFERASE; IN-VITRO CHARACTERIZATION; BIOSYNTHETIC-PATHWAY; DIRECTED EVOLUTION; FLEXIBLE GLYCOSYLTRANSFERASE; ANTIBIOTIC BIOSYNTHESIS; HETEROLOGOUS EXPRESSION; SUBSTRATE-SPECIFICITY; STRUCTURAL INSIGHTS; CRYSTAL-STRUCTURE;
D O I：
10.1016/j.phytochem.2009.05.019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The bioactivity of many natural products produced by microorganisms can be attributed to their sugar substituents. These substituents are transferred as nucleotide-activated sugars to an aglycon by glycosyl-transferases. Engineering these enzymes can broaden their substrate specificity and can therefore have an impact on the bioactivity of the secondary metabolites. In this review we present the generation of a glycosyltransferase gene toolbox which contains more than 70 bacterial glycosyltransferases to date. Investigations of the function, specificity and structure of these glycosyltransferases help to understand the great potential of these enzymes for natural product biosynthesis. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1812 / 1821
页数：10
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