Protective Efficacy of Inactivated Vaccine against SARS-CoV-2 Infection in Mice and Non-Human Primates

被引：15

作者：

Yao, Yan-Feng ^{[1
]}

Wang, Ze-Jun ^{[2
]}

Jiang, Ren-Di ^{[1
,3
]}

Hu, Xue ^{[1
]}

Zhang, Hua-Jun ^{[1
]}

Zhou, Yi-Wu ^{[4
]}

Gao, Ge ^{[1
]}

Chen, Ying ^{[1
,3
]}

Peng, Yun ^{[1
]}

Liu, Mei-Qin ^{[1
,3
]}

Zhang, Ya-Nan ^{[1
,3
]}

Min, Juan ^{[1
]}

Lu, Jia ^{[2
]}

Gao, Xiao-Xiao ^{[1
]}

Guo, Jing ^{[2
]}

Peng, Cheng ^{[1
]}

Shen, Xu-Rui ^{[1
,3
]}

Li, Qian ^{[1
,3
]}

Zhao, Kai ^{[1
,3
]}

Yang, Lian ^{[5
]}

Wan, Xin ^{[2
]}

Zhang, Bo ^{[1
]}

Wang, Wen-Hui ^{[2
]}

Wu, Jia ^{[1
]}

Zhou, Peng ^{[1
]}

Yang, Xing-Lou ^{[1
]}

Shen, Shuo ^{[2
]}

Shan, Chao ^{[1
]}

Yuan, Zhi-Ming ^{[1
]}

Shi, Zheng-Li ^{[1
]}

机构：

[1] Chinese Acad Sci, Ctr Biosafety Megasci, Wuhan Inst Virol, Wuhan 430071, Peoples R China

[2] Wuhan Inst Biol Prod Co Ltd, Wuhan 430024, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[4] Huazhong Univ Sci & Technol, Dept Forens Med, Tongji Med Coll, Wuhan 430074, Peoples R China

[5] Huazhong Univ Sci & Technol, Dept Radiol, Tongji Med Coll, Union Hosp, Wuhan 430074, Peoples R China

来源：

VIROLOGICA SINICA | 2021年 / 36卷 / 05期

基金：

国家重点研发计划;

关键词：

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Coronavirus disease 2019 (COVID-19); Inactivated vaccine; Transgenic mouse; Non-human primate;

D O I：

10.1007/s12250-021-00376-w

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The ongoing coronavirus disease 2019 (COVID-19) pandemic caused more than 96 million infections and over 2 million deaths worldwide so far. However, there is no approved vaccine available for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the disease causative agent. Vaccine is the most effective approach to eradicate a pathogen. The tests of safety and efficacy in animals are pivotal for developing a vaccine and before the vaccine is applied to human populations. Here we evaluated the safety, immunogenicity, and efficacy of an inactivated vaccine based on the whole viral particles in human ACE2 transgenic mouse and in non-human primates. Our data showed that the inactivated vaccine successfully induced SARS-CoV-2-specific neutralizing antibodies in mice and non-human primates, and subsequently provided partial (in low dose) or full (in high dose) protection of challenge in the tested animals. In addition, passive serum transferred from vaccine-immunized mice could also provide full protection from SARS-CoV-2 infection in mice. These results warranted positive outcomes in future clinical trials in humans.

引用

页码：879 / 889

页数：11

共 35 条

[1] Enhancement of Zika virus pathogenesis by preexisting antiflavivirus immunity [J].

Bardina, Susana V. ;

Bunduc, Paul ;

Tripathi, Shashank ;

Duehr, James ;

Frere, Justin J. ;

Brown, Julia A. ;

Nachbagauer, Raffael ;

Foster, Gregory A. ;

Krysztof, David ;

Tortorella, Domenico ;

Stramer, Susan L. ;

Garcia-Sastre, Adolfo ;

Krammer, Florian ;

Lim, Jean K. .

SCIENCE, 2017, 356 (6334) :175-180

[2] Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies [J].

Baum, Alina ;

Fulton, Benjamin O. ;

Wloga, Elzbieta ;

Copin, Richard ;

Pascal, Kristen E. ;

Russo, Vincenzo ;

Giordano, Stephanie ;

Lanza, Kathryn ;

Negron, Nicole ;

Ni, Min ;

Wei, Yi ;

Atwal, Gurinder S. ;

Murphy, Andrew J. ;

Stahl, Neil ;

Yancopoulos, George D. ;

Kyratsous, Christos A. .

SCIENCE, 2020, 369 (6506) :1014-+

[3] Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman Primates [J].

Corbett, Kizzmekia S. ;

Flynn, Barbara ;

Foulds, Kathryn E. ;

Francica, Joseph R. ;

Boyoglu-Barnum, Seyhan ;

Werner, Anne P. ;

Flach, Britta ;

O'Connell, Sarah ;

Bock, Kevin W. ;

Minai, Mahnaz ;

Nagata, Bianca M. ;

Andersen, Hanne ;

Martinez, David R. ;

Noe, Amy T. ;

Douek, Naomi ;

Donaldson, Mitzi M. ;

Nji, Nadesh N. ;

Alvarado, Gabriela S. ;

Edwards, Darin K. ;

Flebbe, Dillon R. ;

Lamb, Evan ;

Doria-Rose, Nicole A. ;

Lin, Bob C. ;

Louder, Mark K. ;

O'Dell, Sijy ;

Schmidt, Stephen D. ;

Phung, Emily ;

Chang, Lauren A. ;

Yap, Christina ;

Todd, John-Paul M. ;

Pessaint, Laurent ;

Van Ry, Alex ;

Browne, Shanai ;

Greenhouse, Jack ;

Putman-Taylor, Tammy ;

Strasbaugh, Amanda ;

Campbell, Tracey-Ann ;

Cook, Anthony ;

Dodson, Alan ;

Steingrebe, Katelyn ;

Shi, Wei ;

Zhang, Yi ;

Abiona, Olubukola M. ;

Wang, Lingshu ;

Pegu, Amarendra ;

Yang, Eun Sung ;

Leung, Kwanyee ;

Zhou, Tongqing ;

Teng, I-Ting ;

Widge, Alicia .

NEW ENGLAND JOURNAL OF MEDICINE, 2020, 383 (16) :1544-1555

[4]

Corbett Kizzmekia S, 2020, bioRxiv, DOI [10.1038/s41586-020-2622-0, 10.1101/2020.06.11.145920]

[5] Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR (Publication with Expression of Concern) [J].

Corman, Victor M. ;

Landt, Olfert ;

Kaiser, Marco ;

Molenkamp, Richard ;

Meijer, Adam ;

Chu, Daniel K. W. ;

Bleicker, Tobias ;

Bruenink, Sebastian ;

Schneider, Julia ;

Schmidt, Marie Luisa ;

Mulders, Daphne G. J. C. ;

Haagmans, Bart L. ;

van der Veer, Bas ;

van den Brink, Sharon ;

Wijsman, Lisa ;

Goderski, Gabriel ;

Romette, Jean-Louis ;

Ellis, Joanna ;

Zambon, Maria ;

Peiris, Malik ;

Goossens, Herman ;

Reusken, Chantal ;

Koopmans, Marion P. G. ;

Drosten, Christian .

EUROSURVEILLANCE, 2020, 25 (03) :23-30

[6] Approaches and Challenges in SARS-CoV-2 Vaccine Development [J].

Dagotto, Gabriel ;

Yu, Jingyou ;

Barouch, Dan H. .

CELL HOST & MICROBE, 2020, 28 (03) :364-370

[7] Effectiveness of convalescent plasma therapy in severe COVID-19 patients [J].

Duan, Kai ;

Liu, Bende ;

Li, Cesheng ;

Zhang, Huajun ;

Yu, Ting ;

Qu, Jieming ;

Zhou, Min ;

Chen, Li ;

Meng, Shengli ;

Hu, Yong ;

Peng, Cheng ;

Yuan, Mingchao ;

Huang, Jinyan ;

Wang, Zejun ;

Yu, Jianhong ;

Gao, Xiaoxiao ;

Wang, Dan ;

Yu, Xiaoqi ;

Li, Li ;

Zhang, Jiayou ;

Wu, Xiao ;

Li, Bei ;

Xu, Yanping ;

Chen, Wei ;

Peng, Yan ;

Hu, Yeqin ;

Lin, Lianzhen ;

Liu, Xuefei ;

Huang, Shihe ;

Zhou, Zhijun ;

Zhang, Lianghao ;

Wang, Yue ;

Zhang, Zhi ;

Deng, Kun ;

Xia, Zhiwu ;

Gong, Qin ;

Zhang, Wei ;

Zheng, Xiaobei ;

Liu, Ying ;

Yang, Huichuan ;

Zhou, Dongbo ;

Yu, Ding ;

Hou, Jifeng ;

Shi, Zhengli ;

Chen, Saijuan ;

Chen, Zhu ;

Zhang, Xinxin ;

Yang, Xiaoming .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2020, 117 (17) :9490-9496

[8] Development of an inactivated vaccine candidate for SARS-CoV-2 [J].

Gao, Qiang ;

Bao, Linlin ;

Mao, Haiyan ;

Wang, Lin ;

Xu, Kangwei ;

Yang, Minnan ;

Li, Yajing ;

Zhu, Ling ;

Wang, Nan ;

Lv, Zhe ;

Gao, Hong ;

Ge, Xiaoqin ;

Kan, Biao ;

Hu, Yaling ;

Liu, Jiangning ;

Cai, Fang ;

Jiang, Deyu ;

Yin, Yanhui ;

Qin, Chengfeng ;

Li, Jing ;

Gong, Xuejie ;

Lou, Xiuyu ;

Shi, Wen ;

Wu, Dongdong ;

Zhang, Hengming ;

Zhu, Lang ;

Deng, Wei ;

Li, Yurong ;

Lu, Jinxing ;

Li, Changgui ;

Wang, Xiangxi ;

Yin, Weidong ;

Zhang, Yanjun ;

Qin, Chuan .

SCIENCE, 2020, 369 (6499) :77-+

[9] The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2 [J].

Gorbalenya, Alexander E. ;

Baker, Susan C. ;

Baric, Ralph S. ;

de Groot, Raoul J. ;

Drosten, Christian ;

Gulyaeva, Anastasia A. ;

Haagmans, Bart L. ;

Lauber, Chris ;

Leontovich, Andrey M. ;

Neuman, Benjamin W. ;

Penzar, Dmitry ;

Perlman, Stanley ;

Poon, Leo L. M. ;

Samborskiy, Dmitry V. ;

Sidorov, Igor A. ;

Sola, Isabel ;

Ziebuhr, John .

NATURE MICROBIOLOGY, 2020, 5 (04) :536-544

[10] Biological challenges and technological opportunities for respiratory syncytial virus vaccine development [J].

Graham, Barney S. .

IMMUNOLOGICAL REVIEWS, 2011, 239 :149-166

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