Spontaneous single-channel activity of neuronal TRP5 channel recombinantly expressed in HEK293 cells

被引：32

作者：

Yamada, H

Wakamori, M ^{[1
]}

Hara, Y

Takahashi, Y

Konishi, K

Imoto, K

Mori, Y

机构：

[1] Natl Inst Physiol Sci, Dept Informat Physiol, Okazaki, Aichi 4448585, Japan

[2] Tohoku Gakuin Univ, Fac Liberal Arts, Lab Neuroinformat Sci, Sendai, Miyagi 9813193, Japan

来源：

NEUROSCIENCE LETTERS | 2000年 / 285卷 / 02期

基金：

日本学术振兴会;

关键词：

cation channel; receptor-activated channel; transient receptor potential protein; patch clamp; single-channel recording; HEK293; cell;

D O I：

10.1016/S0304-3940(00)01033-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Mammalian homologues of the Drosophila transient receptor potential (trp) protein (TRP) form Ca2+ permeable cation channels activated in response to stimulation of G-protein-coupled receptors. Establishing biophysical characteristics of basal TRP activity is of great importance in understanding modulatory processes, which underlie enhancement of TRP activity via receptor stimulation. We have examined spontaneous activity of the TRP5 channel recombinantly expressed in human embryonic kidney cells, using the conventional whole-cell mode of the patch-damp technique in a low-Ca2+ external solution. The unitary Na+ conductance of the TRP5 channel was linear, being 47.6 pS. By contrast, the open probability of the TRP5 channel showed a voltage-dependent decrease below -50 mV. These biophysical properties are important hallmarks in distinguishing the TRP5 channel in native neuronal preparations, whose spontaneous activity may contribute to control of resting membrane potentials and generation of action potentials. (C) 2000 Elsevier Science ireland Ltd. All rights reserved.

引用

页码：111 / 114

页数：4

共 26 条

[1] Contribution of Ca2+-activated K+ channels and non-selective cation channels to membrane potential of pulmonary arterial smooth muscle cells of the rabbit [J].