NMR studies on metal complexes of DNA oligomers

被引：7

作者：

Andersen, B ^{[1
]}

Sletten, E ^{[1
]}

机构：

[1] Univ Bergen, Dept Chem, N-5007 Bergen, Norway

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2000年 / 79卷 / 1-4期

关键词：

nuclear magnetic resonance spectroscopy; oligonucleotides; metal complexes; transplatin; cross-links;

D O I：

10.1016/S0162-0134(99)00230-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A short overview of NMR spectroscopic applications for the study of metal ion complexes of DNA oligomers is presented. One typical example is given to illustrate the scope of the methods: the NMR structure of a trans-DDP interstrand cross-linked duplex, d(CTCCTG*TGTCTC) .d(GAGATA*AGGAG). The solution structure of this double-stranded DNA oligonucleotide, containing a trans-diammineplatinum(II) interstrand cross-link, was determined using two-dimensional nuclear magnetic resonance (2D NMR) and NOE-restrained molecular dynamics and energy minimization refinement. The duplex is a non-palindromic 12/11-mer with a missing central residue in the lower strand and in addition it contains a GT mismatched base pair. The analysis indicated that an interstrand cross-link is established between G6-N7 of the upper strand and A18-N1 of the lower strand. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：353 / 358

页数：6

共 28 条

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