Evaluation of viral concentration methods for SARS-CoV-2 recovery from wastewaters

被引：105

作者：

Angelica Barril, Patricia ^{[1
,2
]}

Alfredo Pianciola, Luis ^{[3
]}

Mazzeo, Melina ^{[3
]}

Julia Ousset, Maria ^{[4
,5
]}

Virginia Jaureguiberry, Maria ^{[1
]}

Alessandrello, Mauricio ^{[1
]}

Sanchez, Gloria ^{[6
]}

Martin Oteiza, Juan ^{[1
,2
]}

机构：

[1] Ctr Invest & Asistencia Tecn Ind CIATI, Lab Microbiol Alimentos, Expedicionarios Desierto 310, RA-8309 Centenario, Neuquen, Argentina

[2] Consejo Nacl Invest Cient & Tecn CONICET, Buenos Aires, DF, Argentina

[3] Minist Salud Prov Neuquen, Lab Cent Mg Luis Alfredo Pianciola, Gregorio Martinez 65, RA-8300 Neuquen, Argentina

[4] Univ Nacl Comahue, Ctr Invest Toxicol Ambiental & Agrobiotecnol Coma, RA-1400 Buenos Aires, DF, Argentina

[5] Univ Nacl Comahue, Ctr Invest Toxicol Ambiental & Agrobiotecnol Coma, RA-8300 Neuquen, Argentina

[6] Consejo Super Invest Cient IATA CSIC, Inst Agroquim & Tecnol Alimentos, Dept Tecnol Conservat & Seguridad Alimentaria, C Catedrat Agustin Escardino Benlloch 7, Valencia 46980, Spain

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 2021年 / 756卷

关键词：

COVID-19; surveillance; Sewage; Wastewater-based epidemiology; Viral concentration methods; RAW SEWAGE; VIRUSES; CORONAVIRUSES; TRANSMISSION; WATER; FOOD;

D O I：

10.1016/j.scitotenv.2020.144105

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Wastewater-based epidemiology (WBE) is a useful tool that has the potential to act as a complementary approach to monitor the presence of SARS-CoV-2 in the community and as an early alarm system for COVID-19 outbreak. Many studies reported low concentrations of SARS-CoV-2 in sewage and also revealed the need for methodological validation for enveloped viruses concentration in wastewater. The aim of this study was to evaluate different methodologies for the concentration of viruses in wastewaters and to select and improve an option that maximizes the recovery of SARS-CoV-2. A total of 11 concentration techniques based on different principles were evaluated: adsorption-elution protocols with negatively charged membranes followed by polyethylene glycol (PEG) precipitation (Methods 1-2), PEG precipitation (Methods 3-7), aluminum polychloride (PAC) flocculation (Method 8), ultrafiltration (Method 9), skim milk flocculation (Method 10) and adsorption-elution with negatively charged membrane followed by ultraliltration (Method 11). To evaluate the performance of these concentration techniques, feline calicivinis (FCV) was used as a process control in order to avoid the risk associated with handling SARS-CoV-2. Two protocols, one based on PEG precipitation and the other on PAC flocculation, showed high efficiency for FCV recovery from wastewater (622% and 45.0%, respectively). These two methods were then tested for the specific recovery of SARS-CoV-2. Both techniques could recover SARS-CoV-2 from wastewater, PAC flocculation showed a lower limit of detection (4.3 x 10(2) GC/mL) than PEG precipitation (43 x 10(3) GC/mL). This work provides a critical overview of current methods used for virus concentration in wastewaters and the analysis of sensitivity for the specific recovery of SARS-CoV-2 in sewage. The data obtained here highlights the viability of WBE for the surveillance of COVID-19 infections in the community. (C) 2020 Elsevier B.V. All rights reserved.

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页数：7

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