A Two-Layer Gold Surface with Improved Surface Enhancement for Spectro-Electrochemistry Using Surface-Enhanced Infrared Absorption Spectroscopy

被引：17

作者：

Nowak, C. ^{[1
]}

Luening, C. ^{[1
]}

Knoll, W. ^{[2
]}

Naumann, R. L. C. ^{[1
]}

机构：

[1] Max Planck Inst Polymer Res, D-55128 Mainz, Germany

[2] Tech Gate Vienna, Austrian Res Ctr GmbH, A-1220 Vienna, Austria

来源：

APPLIED SPECTROSCOPY | 2009年 / 63卷 / 09期

关键词：

Surface-enhanced infrared absorption spectroscopy; SEIRAS; Cytochrome c; Spectro-electrochemistry; Gold nanoparticles; CYTOCHROME-C-OXIDASE; MONOLAYER; ELECTRODE; PARTICLES; SPECTRA; FILMS; IR;

D O I：

10.1366/000370209789379330

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

A two-layer gold surface is developed for use with surface-enhanced infrared absorption spectroscopy (SEIRAS) consisting of a conducting underlayer onto which An nanoparticles (AuNPs) are grown by self-catalyzed electroless deposition. AuNPs are grown on protruding substructures of the 25 nm thin underlayer. The enhancement factor of the two-layer gold surface is controlled by the growth conditions. Cytochrome c adsorbed to a self-assembled monolayer of mercaptoethanol is used as a reference system. Under optimum conditions the absorbance of the amide I band is increased by a factor of 5 versus the classical SEIRAS surface. Reversible reduction/oxidation of cytochrome c on the two-layer gold surface is shown to take place by cyclic voltammetry.

引用

页码：1068 / 1074

页数：7

共 19 条

[1]

Aroca R., 2006, SURFACE ENHANCED VIB

[2] Oriented attachment and membrane reconstitution of his-tagged cytochrome c oxidase to a gold electrode:: In situ monitoring by surface-enhanced infrared absorption spectroscopy [J].