Establishment of a novel testicular cell line from sterlet Acipenser ruthenus and evaluation of its applications

被引：4
|
作者：
Sun, Ai [1 ,2 ]
Zhu, Hua [1 ,2 ]
Dong, Ying [1 ,2 ]
Wang, Wei [1 ,2 ]
Hu, Hong Xia [1 ,2 ]
机构：
[1] Beijing Fisheries Res Inst, Beijing 100068, Peoples R China
[2] Minist Sci & Technol China, Natl Freshwater Fisheries Engn Technol Res Ctr, Lab Biol Technol & Breeding, Beijing Key Lab Fishery Biotechnol, Beijing, Peoples R China
来源：
JOURNAL OF FISH BIOLOGY | 2019年 / 94卷 / 05期
基金：
中国国家自然科学基金;
关键词：
Acipenser ruthenus; cell line; gene expression; sturgeon; testis; transfection; 11-BETA-HYDROXYSTEROID DEHYDROGENASE; DIMORPHIC EXPRESSION; GERM-CELLS; FISH; DIFFERENTIATION; CONSERVATION; DMRT1; GONAD;
D O I：
10.1111/jfb.13855
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
In this study, a cell line, designated as Acipenser ruthenus testis (ART), was successfully established from testis tissues of the sterlet Acipenser ruthenus and characterized by studying and comparing the expression of specific genes between the cell line and the parent gonad tissues. The results suggested that the developed ART cell line was composed of a mixture of germ cells and somatic cells. Ploidy analysis indicated that the cell line exhibited a high degree of genetic stability and that the cells remained in a good proliferating state after being subcultured to passage 80.
引用
收藏
页码：804 / 809
页数：6
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