Effect of organic loading rate on the anaerobic digestion of swine waste with biochar addition

The aim of this study was to investigate the impact of biochar addition on the mesophilic semi-continuous anaerobic digestion of swine waste with a focus on the effects of the organic loading rate (OLR) on biogas production, methane yield, total volatile fatty acids (TVFA), alkalinity, ammonium, volatile solids (VS) removal efficiency and process stability. Four reactors, two with amended biochar (R1 and R2) and two without biochar addition as controls (R3 and R4), were operated at OLRs in the range of 2-7 g VS/(L d), which corresponded to hydraulic retention times (HRTs) in the range of 7-2 days, respectively. The addition of biochar initially caused an increase in the generation of biogas and methane when compared to the control reactors when the process operated at OLRs of 2 and 3 g VS/(L d). This behaviour could be attributed to the presence of several trace elements (such as Fe, Co, Ni and Mn) in the biochar, which are involved in the action of acetyl-CoA synthase and methyl coenzyme M reductase to catalyse key metabolic steps, especially the methanogenic stage. The pH, alkalinity, TVFA and TVFA/Alkalinity ratio values for the effluents remained within the optimal ranges for the anaerobic digestion process. It was also found that the increase in OLR in the range of 2-5 g VS/(L d) determined a proportional increase in the VS removal rate. However, when the OLR increased up to 7 g VS/(L d), a drastic decrease in the VS removal rate was found for the control reactors. Biochar amendment contributed to a more balanced state of the anaerobic process, preventing biomass washout.