Capillary zone electrophoresis for bottom-up analysis of complex proteomes

被引：38

作者：

Sun, Liangliang ^{[1
]}

Zhu, Guijie ^{[1
]}

Yan, Xiaojing ^{[1
]}

Zhang, Zhenbin ^{[1
]}

Wojcik, Roza ^{[1
]}

Champion, Matthew M. ^{[1
]}

Dovichi, Norman J. ^{[1
]}

机构：

[1] Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46557 USA

来源：

PROTEOMICS | 2016年 / 16卷 / 02期

基金：

美国国家卫生研究院;

关键词：

Bottom-up proteomics; Capillary electrophoresis; Cell biology; Electrokinetically pumped nanospray; Technology; TANDEM MASS-SPECTROMETRY; ELECTROSPRAY-IONIZATION INTERFACE; FLOW NANOSPRAY INTERFACE; DYNAMIC PH JUNCTION; 264.7 CELL LYSATE; PEPTIDE IDENTIFICATIONS; QUANTITATIVE PROTEOMICS; SEPARATION TECHNIQUES; SENSITIVITY; MIXTURES;

D O I：

10.1002/pmic.201500339

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary zone electrophoresis (CZE) is emerging as a useful tool in proteomic analysis. Interest arises from dramatic improvements in performance that result from improvements in the background electrolyte used for the separation, the incorporation of advanced sample injection methods, the development of robust and sensitive electrospray interfaces, and the coupling with Orbitrap mass spectrometers with high resolution and sensitivity. The combination of these technologies produces performance that is rapidly approaching the performance of UPLC-based methods for microgram samples and exceeds the performance of UPLC-based methods for mid-to low nanogram samples. These systems now produce over 10 000 peptide IDs in a single 100-min analysis of the HeLa proteome.

引用

页码：188 / 196

页数：9

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