Super-Resolution Imaging with Small Organic Fluorophores

被引：313
作者：
Heilemann, Mike [1 ]
van de Linde, Sebastian [1 ]
Mukherjee, Anindita [1 ]
Sauer, Markits [1 ]
机构：
[1] Univ Bielefeld, D-33615 Bielefeld, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 37期
关键词：
diffraction limit; dyes/pigments; live-cell imaging; photoswitching; super-resolution imaging; GROUND-STATE-DEPLETION; FLUORESCENCE NANOSCOPY; AROMATIC THIOLS; MICROSCOPY; MOLECULES; DYES; GLUTATHIONE; OXIDATION; PROTEINS; BREAKING;
D O I：
10.1002/anie.200902073
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
What's your color? A universal and facile method for reversible photoswitching of commercially available Alexa Fluor and ATTO dyes spanning the entire visible range yields an optical resolution of approximately 20 nm. The intriguing simplicity of the method facilitates applications and opens avenues for multicolor super-resolution imaging in fixed and living cells. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：6903 / 6908
页数：6
相关论文
共 41 条
  • [11] Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: Numerical analysis and application to the fluorescent protein Dronpa
    Dedecker, Peter
    Hotta, Jun-Ichi
    Flors, Cristina
    Sliwa, Michel
    Uji-, Hiroshi, I
    Roeffaers, Maarten B. J.
    Ando, Ryoko
    Mizuno, Hideaki
    Miyawaki, Atsushi
    Hofkens, Johan
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (51) : 16132 - 16141
  • [12] Methods for visualizing RNA processing and transport pathways in living cells
    Dirks, RW
    Molenaar, C
    Tanke, HJ
    [J]. HISTOCHEMISTRY AND CELL BIOLOGY, 2001, 115 (01) : 3 - 11
  • [13] Fluorescent probes for super-resolution imaging in living cells
    Fernandez-Suarez, Marta
    Ting, Alice Y.
    [J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2008, 9 (12) : 929 - 943
  • [14] Fluorescence nanoscopy by ground-state depletion and single-molecule return
    Foelling, Jonas
    Bossi, Mariano
    Bock, Hannes
    Medda, Rebecca
    Wurm, Christian A.
    Hein, Birka
    Jakobs, Stefan
    Eggeling, Christian
    Hell, Stefan W.
    [J]. NATURE METHODS, 2008, 5 (11) : 943 - 945
  • [15] Carbocyanine dyes as efficient reversible single-molecule optical switch
    Heilemann, M
    Margeat, E
    Kasper, R
    Sauer, M
    Tinnefeld, P
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (11) : 3801 - 3806
  • [16] Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes
    Heilemann, Mike
    van de Linde, Sebastian
    Schuttpelz, Mark
    Kasper, Robert
    Seefeldt, Britta
    Mukherjee, Anindita
    Tinnefeld, Philip
    Sauer, Markus
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (33) : 6172 - 6176
  • [17] Photoswitches: Key molecules for subdiffraction-resolution fluorescence imaging and molecular quantification
    Heilemann, Mike
    Dedecker, Peter
    Hofkens, Johan
    Sauer, Markus
    [J]. LASER & PHOTONICS REVIEWS, 2009, 3 (1-2) : 180 - 202
  • [18] Photoinduced electron transfer between fluorescent dyes and guanosine residues in DNA-hairpins
    Heinlein, T
    Knemeyer, JP
    Piestert, O
    Sauer, M
    [J]. JOURNAL OF PHYSICAL CHEMISTRY B, 2003, 107 (31) : 7957 - 7964
  • [19] Far-field optical nanoscopy
    Hell, Stefan W.
    [J]. SCIENCE, 2007, 316 (5828) : 1153 - 1158
  • [20] GROUND-STATE-DEPLETION FLUORESCENCE MICROSCOPY - A CONCEPT FOR BREAKING THE DIFFRACTION RESOLUTION LIMIT
    HELL, SW
    KROUG, M
    [J]. APPLIED PHYSICS B-LASERS AND OPTICS, 1995, 60 (05): : 495 - 497
12345