Super-Resolution Imaging with Small Organic Fluorophores

被引：313
作者：
Heilemann, Mike [1 ]
van de Linde, Sebastian [1 ]
Mukherjee, Anindita [1 ]
Sauer, Markits [1 ]
机构：
[1] Univ Bielefeld, D-33615 Bielefeld, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 37期
关键词：
diffraction limit; dyes/pigments; live-cell imaging; photoswitching; super-resolution imaging; GROUND-STATE-DEPLETION; FLUORESCENCE NANOSCOPY; AROMATIC THIOLS; MICROSCOPY; MOLECULES; DYES; GLUTATHIONE; OXIDATION; PROTEINS; BREAKING;
D O I：
10.1002/anie.200902073
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
What's your color? A universal and facile method for reversible photoswitching of commercially available Alexa Fluor and ATTO dyes spanning the entire visible range yields an optical resolution of approximately 20 nm. The intriguing simplicity of the method facilitates applications and opens avenues for multicolor super-resolution imaging in fixed and living cells. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：6903 / 6908
页数：6
相关论文
共 41 条
  • [1] Abbe E., 1873, Arch. Mikrosk. Anat., V9, P413, DOI DOI 10.1007/BF02956173
  • [2] [Anonymous], 2008, ANGEW CHE
  • [3] Multicolor super-resolution imaging with photo-switchable fluorescent probes
    Bates, Mark
    Huang, Bo
    Dempsey, Graham T.
    Zhuang, Xiaowei
    [J]. SCIENCE, 2007, 317 (5845) : 1749 - 1753
  • [4] Imaging intracellular fluorescent proteins at nanometer resolution
    Betzig, Eric
    Patterson, George H.
    Sougrat, Rachid
    Lindwasser, O. Wolf
    Olenych, Scott
    Bonifacino, Juan S.
    Davidson, Michael W.
    Lippincott-Schwartz, Jennifer
    Hess, Harald F.
    [J]. SCIENCE, 2006, 313 (5793) : 1642 - 1645
  • [5] Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
    Bossi, Mariano
    Foelling, Jonas
    Belov, Vladimir N.
    Boyarskiy, Vadim P.
    Medda, Rebecca
    Egner, Alexander
    Eggeling, Christian
    Schoenle, Andreas
    Hell, Stefan W.
    [J]. NANO LETTERS, 2008, 8 (08) : 2463 - 2468
  • [6] Transient-state and steady-state kinetics of the oxidation of aliphatic and aromatic thiols by horseradish peroxidase
    Burner, U
    Obinger, C
    [J]. FEBS LETTERS, 1997, 411 (2-3) : 269 - 274
  • [7] Kinetics of oxidation of aliphatic and aromatic thiols by myeloperoxidase compounds I and II
    Burner, U
    Jantschko, W
    Obinger, C
    [J]. FEBS LETTERS, 1999, 443 (03) : 290 - 296
  • [8] Spectroscopic study and evaluation of red-absorbing fluorescent dyes
    Buschmann, V
    Weston, KD
    Sauer, M
    [J]. BIOCONJUGATE CHEMISTRY, 2003, 14 (01) : 195 - 204
  • [9] Site-specific labeling of proteins with small molecules in live cells
    Chen, I
    Ting, AY
    [J]. CURRENT OPINION IN BIOTECHNOLOGY, 2005, 16 (01) : 35 - 40
  • [10] DITHIOTHREITOL NEW PROTECTIVE REAGENT FOR SH GROUPS
    CLELAND, WW
    [J]. BIOCHEMISTRY, 1964, 3 (04) : 480 - &
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