Optimization of Ultrasound-Assisted Extraction of Natural Antioxidants from the Osmanthus fragrans Flower

被引:20
作者
Li, An-Na [1 ]
Li, Sha [2 ]
Li, Ya [1 ]
Xu, Dong-Ping [1 ]
Li, Hua-Bin [1 ]
机构
[1] Sun Yat Sen Univ, Sch Publ Hlth, Guangdong Prov Key Lab Food Nutr & Hlth, Guangzhou 510080, Guangdong, Peoples R China
[2] Univ Hong Kong, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China
基金
中国国家自然科学基金;
关键词
Osmanthus fragrans; flower; antioxidant; ultrasound-assisted extraction; response surface methodology; TOTAL PHENOLIC CONTENTS; OXIDATIVE STRESS; GREEN TEA; CAPACITIES; FRACTIONS; CANCER; LIPIDS;
D O I
10.3390/molecules21020218
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An ultrasound-assisted extraction (UAE) method was developed to extract natural antioxidants from the Osmanthus fragrans flower. The effect of UAE on antioxidant activity of the extract from the Osmanthus fragrans flower was studied using a Trolox equivalent antioxidant capacity (TEAC) assay. Optimization conditions were firstly determined using a single-factor experiment, and response surface methodology was then used to evaluate interaction of several experimental parameters. Analysis of the coefficient of determination showed that second-order polynomial models produced a highly satisfactory fitting of the experimental data with regard to TEAC values (R-2 = 0.9829, p < 0.0001). The optimal conditions were 39.1% ethanol, and extraction for 35.2 min at 59.4 degrees C. Under these conditions, the maximum TEAC value was 584.9 +/- 6.0 mol Trolox/g DW, which was higher than those obtained by the conventional extracting method (486.4 +/- 12.6 mol Trolox/g DW) and the Soxhlet extraction method (339.1 +/- 16.2 mol Trolox/g DW). The crude extract obtained could be used either as a food additive or in pharmaceuticals for the prevention and treatment of diseases caused by oxidative stress.
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页数:11
相关论文
共 38 条
[1]   Extraction of lipids from microalgae by ultrasound application: Prospection of the optimal extraction method [J].
Araujo, Glacio S. ;
Matos, Leonardo J. B. L. ;
Fernandes, Jader O. ;
Cartaxo, Samuel J. M. ;
Goncalves, Luciana R. B. ;
Fernandes, Fabiano A. N. ;
Farias, Wladimir R. L. .
ULTRASONICS SONOCHEMISTRY, 2013, 20 (01) :95-98
[2]   Free radicals, oxidative stress, and antioxidants in human health and disease [J].
Aruoma, OI .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1998, 75 (02) :199-212
[3]   Oxidative stress in plants [J].
Bartosz, G .
ACTA PHYSIOLOGIAE PLANTARUM, 1997, 19 (01) :47-64
[4]   FRUIT, VEGETABLES, AND CANCER PREVENTION - A REVIEW OF THE EPIDEMIOLOGIC EVIDENCE [J].
BLOCK, G ;
PATTERSON, B ;
SUBAR, A .
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 1992, 18 (01) :1-29
[5]   ON THE EXPERIMENTAL ATTAINMENT OF OPTIMUM CONDITIONS [J].
BOX, GEP ;
WILSON, KB .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1951, 13 (01) :1-45
[6]   Optimization of Ultrasound-Assisted Extraction of Natural Antioxidants from Sugar Apple (Annona squamosa L.) Peel Using Response Surface Methodology [J].
Deng, Gui-Fang ;
Xu, Dong-Ping ;
Li, Sha ;
Li, Hua-Bin .
MOLECULES, 2015, 20 (11) :20448-20459
[7]   Determination of antioxidant property and their lipophilic and hydrophilic phenolic contents in cereal grains [J].
Deng, Gui-Fang ;
Xu, Xiang-Rong ;
Guo, Ya-Jun ;
Xia, En-Qin ;
Li, Sha ;
Wu, Shan ;
Chen, Feng ;
Ling, Wen-Hua ;
Li, Hua-Bin .
JOURNAL OF FUNCTIONAL FOODS, 2012, 4 (04) :906-914
[8]   Investigation on ultrasound-assisted extraction of salvianolic acid B from Salvia miltiorrhiza root [J].
Dong, Juane ;
Liu, Yuanbai ;
Liang, Zongsuo ;
Wang, Weiling .
ULTRASONICS SONOCHEMISTRY, 2010, 17 (01) :61-65
[9]   Plant polyphenols in cancer and heart disease: implications as nutritional antioxidants [J].
Duthie, GG ;
Duthie, SJ ;
Kyle, JAM .
NUTRITION RESEARCH REVIEWS, 2000, 13 (01) :79-106
[10]   Antioxidant capacities and total phenolic contents of 62 fruits [J].
Fu, Li ;
Xu, Bo-Tao ;
Xu, Xiang-Rong ;
Gan, Ren-You ;
Zhang, Yuan ;
Xia, En-Qin ;
Li, Hua-Bin .
FOOD CHEMISTRY, 2011, 129 (02) :345-350