Two new pregnane glycosides from Reineckia carnea

Two new pregnane glycosides (1-2) and two known cholestane glycosides (3-4) were obtained from the whole plant Reineckia carnea. Their structures were characterized by physicochemical properties and spectroscopic methods, as (17, 20-S-trans)-5 beta-pregn-16-en-1 beta, 3 beta-ol-20-one 1-O-beta-D-xylopyranosyl(1 -> 2)-alpha-L-rhamnopyranoside 3-O-alpha-L-rhamnopyranoside (1), (17,20-S-trans)-pregna-5,16-dien-3 beta-ol-20-one 3-O-beta-D-glucopyranosyl-(1 -> 2)-O-[beta-D-xylopyranosyl-(1 -> 3)]-O-beta-D-glucopyranosyl-(1 -> 4)-O-beta-D-galactopyranoside (2), (22S)-cholest-1 beta, 3 beta, 16 beta, 22-tetraol 1-O-beta-D-glucopyrannoside 16-O-beta-D-glucopyrannoside (3), and (22S)-cholest-1 beta, 3 beta, 16 beta, 22-tetraol 1-O-alpha-L-rhamnopyranosyl-(1 -> 2)-beta-D-glucopyrannoside 16-O-beta-D-glucopyrannoside (4). In addition, their cytotoxic activity against human A549 tumor cells was evaluated by MTT method with 1-4 exhibited cytotoxicity with IC50 values of 87.7 mu mol l (1), 41.2 mu mol l (1), 46.6 mu mol l (1), and 49.5 mu mol l (1), respectively. (C) 2015 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.