Simultaneous extraction and HPLC determination of 3-indole butyric acid and 3-indole acetic acid in pea plant by using ionic liquid-modified silica as sorbent

被引：26

作者：

Sheikhian, Leila ^{[1
]}

Bina, Sedigheh ^{[2
]}

机构：

[1] Islamic Azad Univ, Dept Chem, Kazerun Branch, Kazerun, Iran

[2] Islamic Azad Univ, Dept Chem, Firoozabad Branch, Firoozabad, Iran

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2016年 / 1009卷

关键词：

Extraction; HPLC; 3-Indole acetic acid; 3-Indole butyric acid; Ionic liquid-modified silica gel; SOLID-PHASE EXTRACTION; INDOLE-3-BUTYRIC ACID; CAPILLARY-ELECTROPHORESIS; WATER SAMPLES; ABSCISIC-ACID; CHROMATOGRAPHY; SEPARATION; MICROEXTRACTION; SPECTROMETRY; GROWTH;

D O I：

10.1016/j.jchromb.2015.11.047

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, ionic liquid-modified silica was used as sorbent for simultaneous extraction and preconcentration of 3-indole butyric acid and 3-indole acetic acid in pea plants. The effect of some parameters such as pH and ionic strength of sample solution, amount of sorbent, flow rate of aqueous sample solution and eluent solution, concentration of eluent solution, and temperature were studied for each hormone solution. Percent extraction of 3-indole butyric acid and 3-indole acetic acid was strongly affected by pH of aqueous sample solution. Ionic strength of aqueous phase and temperature showed no serious effects on extraction efficiency of studied plant hormones. Obtained breakthrough volume was 200 mL for each of studied hormones. Preconcentration factor for spectroscopic and chromatographic determination of studied hormones was 100 and 4.0 x 10(3) respectively. Each solid sorbent phase was reusable for almost 10 times of extraction/stripping procedure. Relative standard deviations of extraction/stripping processes of 3-indole butyric acid and 3-indole acetic acid were 2.79% and 3.66% respectively. The calculated limit of detections for IBA and IAA were 9.1 x 10(-2) mg L-1 and 1.6 x 10(-1) mg L-1 respectively. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：34 / 43

页数：10

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