Sequence variant (CTAGGG)n in the human telomere favors a G-quadruplex structure containing a G•C•G•C tetrad

被引：119

作者：

Lim, Kah Wai ^{[1
]}

Alberti, Patrizia ^{[2
]}

Guedin, Aurore ^{[2
]}

Lacroix, Laurent ^{[2
]}

Riou, Jean-Francois ^{[2
]}

Royle, Nicola J. ^{[3
]}

Mergny, Jean-Louis ^{[2
]}

Phan, Anh Tuan ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Phys & Math Sci, Div Phys & Appl Phys, Singapore, Singapore

[2] Museum Natl Hist Nat, CNRS, INSERM, UMR 7196,USM 503,U565, Paris, France

[3] Univ Leicester, Dept Genet, Leicester LE1 7RH, Leics, England

来源：

NUCLEIC ACIDS RESEARCH | 2009年 / 37卷 / 18期

基金：

英国医学研究理事会;

关键词：

INTRAMOLECULAR G-QUADRUPLEX; K+ SOLUTION; DNA QUADRUPLEX; TETRAMOLECULAR QUADRUPLEXES; REPEAT; NMR; SUBSTITUTIONS; POTASSIUM; KINETICS; FORMS;

D O I：

10.1093/nar/gkp630

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Short contiguous arrays of variant CTAGGG repeats in the human telomere are unstable in the male germline and somatic cells, suggesting formation of unusual structures by this repeat type. Here, we report on the structure of an intramolecular G-quadruplex formed by DNA sequences containing four human telomeric variant CTAGGG repeats in potassium solution. Our results reveal a new robust antiparallel G-quadruplex fold involving two G-tetrads sandwiched between a G. C base pair and a G center dot C center dot G center dot C tetrad, which could represent a new platform for drug design targeted to human telomeric DNA.

引用

页码：6239 / 6248

页数：10

共 49 条

[1] HUMAN TELOMERES CONTAIN AT LEAST 3 TYPES OF G-RICH REPEAT DISTRIBUTED NON-RANDOMLY [J].

ALLSHIRE, RC ;

DEMPSTER, M ;

HASTIE, ND .

NUCLEIC ACIDS RESEARCH, 1989, 17 (12) :4611-4627

[2] Human telomeric sequence forms a hybrid-type intramolecular G-quadruplex structure with mixed parallel/antiparallel strands in potassium solution [J].

Ambrus, Attila ;

Chen, Ding ;

Dai, Jixun ;

Bialis, Tiffanie ;

Jones, Roger A. ;

Yang, Danzhou .

NUCLEIC ACIDS RESEARCH, 2006, 34 (09) :2723-2735

[3] Length-dependent energetics of (CTG)n and (CAG)n trinucleotide repeats [J].

Amrane, S ;

Saccà, B ;

Mills, M ;

Chauhan, M ;

Klump, HH ;

Mergny, JL .

NUCLEIC ACIDS RESEARCH, 2005, 33 (13) :4065-4077

[4] A novel chair-type G-quadruplex formed by a Bombyx mori telomeric sequence [J].

Amrane, Samir ;

Ang, Rita Wan Lin ;

Tan, Zhong Ming ;

Li, Chun ;

Lim, Joefina Kim Cheow ;

Lim, Jocelyn Mei Wen ;

Lim, Kah Wai ;

Phan, Anh Tuan .

NUCLEIC ACIDS RESEARCH, 2009, 37 (03) :931-938

[5] MECHANISMS UNDERLYING TELOMERE REPEAT TURNOVER, REVEALED BY HYPERVARIABLE VARIANT REPEAT DISTRIBUTION PATTERNS IN THE HUMAN XP/YP TELOMERE [J].

BAIRD, DM ;

JEFFREYS, AJ ;

ROYLE, NJ .

EMBO JOURNAL, 1995, 14 (21) :5433-5443

[6] A K cation-induced conformational switch within a loop spanning segment of a DNA quadruplex containing G-G-G-C repeats [J].

Bouaziz, S ;

Kettani, A ;

Patel, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1998, 282 (03) :637-652

[7] OLIGONUCLEOTIDE INTERACTIONS .3. CIRCULAR DICHROISM STUDIES OF CONFORMATION OF DEOXYOLIGONUCLEOTIDES [J].

CANTOR, CR ;

WARSHAW, MM ;

SHAPIRO, H .

BIOPOLYMERS, 1970, 9 (09) :1059-&

[8]

DAI J, 2007, NUCLEIC ACIDS RES, V42, P481

[9] Structure of the Hybrid-2 type intramolecular human telomeric G-quadruplex in K+ solution:: insights into structure polymorphism of the human telomeric sequence [J].

Dai, Jixun ;

Carver, Megan ;

Punchihewa, Chandanamali ;

Jones, Roger A. ;

Yang, Danzhou .

NUCLEIC ACIDS RESEARCH, 2007, 35 (15) :4927-4940

[10] Targeting telomeres and telomerase [J].

De Cian, Anne ;

Lacroix, Laurent ;

Douarre, Celine ;

Temime-Smaali, Nassima ;

Trentesaux, Chantal ;

Riou, Jean-Francois ;

Mergny, Jean-Louis .

BIOCHIMIE, 2008, 90 (01) :131-155

← 1 2 3 4 5 →