Biotransformation of fluorobiphenyl by Cunninghamella elegans

被引：24

作者：

Amadio, Jessica ^{[1
]}

Murphy, Cormac D. ^{[1
]}

机构：

[1] Univ Coll Dublin, Sch Biomol & Biomed Sci, Ctr Synth & Chem Biol, Dublin 4, Ireland

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2010年 / 86卷 / 01期

关键词：

Fluorine; Biphenyl; Metabolism; F-19; NMR; NUCLEAR-MAGNETIC-RESONANCE; FUNGAL METABOLISM; PHANEROCHAETE-CHRYSOSPORIUM; DEGRADATION; BIPHENYL; FLUORINE;

D O I：

10.1007/s00253-009-2346-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The fungus Cunninghamella elegans is a useful model of human catabolism of xenobiotics. In this paper, the biotransformation of fluorinated biphenyls by C. elegans was investigated by analysis of the culture supernatants with a variety of analytical techniques. 4-Fluorobiphenyl was principally transformed to 4-fluoro-4'-hydroxybiphenyl, but other mono- and dihydroxylated compounds were detected in organic extracts by gas chromatography-mass spectrometry. Additionally, fluorinated water-soluble products were detected by F-19 NMR and were identified as sulphate and beta-glucuronide conjugates. Other fluorobiphenyls (2-fluoro-, 4,4'-difluoro- and 2,3,4,5,6-pentafluoro-biphenyl) were catabolised by C. elegans, yielding mono- and dihydroxylated products, but phase II metabolites were detected from 4,4'-difluorobiphenyl only.

引用

页码：345 / 351

页数：7

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