Distinct distal and proximal p53-binding sites in the MCK promoter govern the transcriptional response to p53

被引:4
作者
Jackson, P
Yardley, G
机构
[1] Oncology Research Centre, Prince of Wales Hospital, Randwick
关键词
p53; muscle creatine kinase; promoter; transcription; DNA binding; gene activation;
D O I
10.1016/S0014-5793(97)00283-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have investigated the functional importance of nucleotide sequence differences between proximal (P-) and distal (D-) p53-binding elements in the MCK promoter, P- and D-elements normally co-operate to permit synergistic promoter activation by p53. Interestingly, we find that P-elements cannot co-operate with each other, In contrast, co-operation between D-binding sites results in levels of p53-induced transcription far higher than those obtained by co-operation between P- and D-elements, These studies imply that distinct D- and P-p53-binding sites in the MCK promoter may dictate the promoter response to p53. (C) 1997 Federation of European Biochemical Societies.
引用
收藏
页码:271 / 274
页数:4
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