Profiling of molecular interactions in real time using acoustic detection

被引：14

作者：

Godber, Benjamin ^{[1
]}

Frogley, Mark ^{[1
]}

Rehak, Marian ^{[1
]}

Sleptsov, Alexander ^{[1
]}

Thompson, Kevin S. J. ^{[1
]}

Uludag, Yildiz ^{[1
]}

Cooper, Matthew A. ^{[1
]}

机构：

[1] Akubio Ltd, U181, Cambridge CB4 0GJ, England

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 9-10期

关键词：

QCM; kinetics; microfluidics; affinity; label-free; binding;

D O I：

10.1016/j.bios.2006.10.019

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Acoustic sensors that exploit resonating quartz crystals to directly detect the binding of an analyte to a receptor are finding increasing utility in the quantification of clinically relevant analytes. We have developed a novel acoustic detection technology, which we term resonant acoustic profiling (RAP (TM)). This technology builds on the fundamental basics of the "quartz crystal microbalance" or "QCM" with several key additional features including two- or four-channel automated sample delivery, in-line referencing and microfluidic sensor 'cassettes' that are pre-coated with easy-to-use surface chemistries. Example applications are described for the quantification of myoglobin concentration and its interaction kinetics, and for the ranking of enzyme-cofactor specificities. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：2382 / 2386

页数：5

共 28 条

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