School climate can be seen as a significant antecedent of student well-being, where various aspects of school climate can have both positive and negative influence on student outcomes, with well-being as one of them. During their student life, university and its environment play a significant role in youth development and both affective, and cognitive well-being. Therefore the aim of this paper is to investigate the connection between school climate and student well-being. Empirical research was done on a sample of 187 students from the University of Zagreb, Croatia. Research results reveal that different aspects of school climate can be associated with student well-being. Justice in school, professor support and safety at school are aspects positively related to well-being, while loneliness during breaks is associated with lower levels of well-being. Disturbances in class, student support, unreasonable expectations, and bullying as aspects of climate were not statistically significantly associated with student well-being.