Spectrophotometric determination of tranexamic acid in dosage forms by derivatization

Tranexamic acid (TA) was determined spectrophotometrically after derivatizing with salicylaldehyde (SA) or 5, 5'-methylene-bis-salicylaldehyde (BSA) at neutral pH in aqueous-methanolic solution. The reaction conditions were optimized and the derivatives absorbed maximally at 410 nm and 412 nm for SA and BSA respectively. The Beee's law was obeyed in the range 7.8-78.6 mu g/ml tranexamic acid, with coefficient of determination of 0.9995 and 0.9998. The methods were applied for the determination of tranexamic acid in pharmaceutical preparations with coefficient of variation 0.76-0.97%.