Single-chamber microbial fuel cells (S-MFCs) with bio-anodes and activated carbon (AC) air-cathodes showed high nitrobenzene (NB) tolerance and NB removal with concomitant electricity production. The maximum power over 25 W m(-3) could be obtained when S-MFCs were operated in the NB loading range of 1.2-6.2 mol m(-3) d(-1), and stable electricity production over 13.7 W m(-3) could be produced in a NB loading range of 1.2-14.7 mol m(-3) d(-1). The present S-MFCs exhibited high NB removal performance with NB removal efficiency over 97% even when the NB loading rate was increased to 17.2 mol m(-3) d(-1). The potential NB reduced product (i.e. aniline) could also be effectively removed from influents. The findings in this study means that single-chamber MFCs assembled with pre-enriched bio-anodes and AC aircathodes could be developed as effective bio-electrochemical systems to remove NB from wastewaters and to harvest energy instead of consuming energy. (C) 2017 Elsevier Ltd. All rights reserved.