Fast decellularization process using supercritical carbon dioxide for trabecular bone

被引：15

作者：

Duarte, Marta M. ^{[1
]}

Ribeiro, Nilza ^{[1
,2
]}

Silva, Ines, V ^{[1
]}

Dias, Juliana R. ^{[3
]}

Alves, Nuno M. ^{[3
]}

Oliveira, Ana L. ^{[1
]}

机构：

[1] Univ Catolica Portuguesa, CBQF Ctr Biotecnol & Quim Fina, Lab Associado, Escola Super Biotecnol, P-4200375 Porto, Portugal

[2] Univ Aveiro, DEMC Dept Engn Mat & Ceram, P-3810193 Aveiro, Portugal

[3] Polytech Inst Leiria, CDRSP Ctr Rapid & Sustainable Prod Dev, P-2430028 Marinha Grande, Portugal

来源：

JOURNAL OF SUPERCRITICAL FLUIDS | 2021年 / 172卷 / 172期

关键词：

Decellularization; Supercritical carbon dioxide; TnBP; Trabecular bone;

D O I：

10.1016/j.supflu.2021.105194

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Decellularization is a process that consists on the removal of immunogenic cellular material from a tissue, so that it can be safely implanted as a functional and bioactive scaffold. Most decellularization protocols rely on the use of harsh chemicals and very long washing processes, leading to severe changes in the ultrastructure and loss of mechanical integrity. To tackle these challenges, supercritical carbon dioxide (scCO2) is herein proposed as an alternative methodology for assisting decellularization of porcine trabecular bone tissue and is combined, for the first time, with Tri(n-butyl) phosphate (TnBP). Histological and DNA analysis revealed that both TnBP and scCO2 were able to extract the DNA content from the scaffolds, being this effect more pronounced in treatments that used TnBP as a co-solvent. The combined protocol led to a decrease in DNA content by at least 90%, demonstrating the potential of this methodology and opening new possibilities for future optimizations.

引用

页数：13

共 62 条

[31] Preparation of acellular scaffold for corneal tissue engineering by supercritical carbon dioxide extraction technology [J].