Determination of cysteine in human plasma by high-performance liquid chromatography and ultraviolet detection after pre-column derivatization with 2-chloro-1-methylpyridinium iodide

被引：218

作者：

Chwatko, G ^{[1
]}

Bald, E ^{[1
]}

机构：

[1] Univ Lodz, Dept Environm Chem, PL-90236 Lodz, Poland

来源：

TALANTA | 2000年 / 52卷 / 03期

关键词：

cysteine; 2-chloro-1-methylpyridinium iodide; derivatization; HPLC; plasma;

D O I：

10.1016/S0039-9140(00)00394-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes the development and validation of a reversed-phase HPLC method with isocratic conditions for the analysis of total cysteine in human plasma. The essential steps in the assay include derivatization of cysteine via thiol group with 2-chloro-1-methylpyridinium iodide (CMPI), separation of the formed cysteine S-pyridinium derivative from endogenous thiol derivatives, usually present in biological samples, by ion-pair reversed-phase HPLC, and ultraviolet detection. Free cystine and protein-bound cysteine are converted to their reduced counterpart by the use of 2-mercaptoethanol. The standard curve is linear over the concentration range from 20 to 300 nmol ml(-1). The recoveries are from 93.9 to 105.90% and imprecision was at most 2.5%. Optimal conditions are determined for all steps of the analytical procedure. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：509 / 515

页数：7

共 15 条

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ANDERSSON A, 1995, CLIN CHEM, V41, P361

[2] PLASMA SULFHYDRYL-CONTAINING AMINO-ACIDS IN PATIENTS WITH CEREBRAL INFARCTION AND IN HYPERTENSIVE SUBJECTS [J].