Shining new light on 3D cell motility and the metastatic process

被引：47

作者：

Provenzano, Paolo P. ^{[1
,2
,3
]}

Eliceiri, Kevin W. ^{[1
,2
]}

Keely, Patricia J. ^{[1
,2
,3
]}

机构：

[1] Univ Wisconsin, Mol Biol Lab, Lab Opt & Computat Instrumentat, Madison, WI 53706 USA

[2] Univ Wisconsin, Dept Biomed Engn, Madison, WI 53706 USA

[3] Univ Wisconsin, Dept Pharmacol, Madison, WI 53706 USA

来源：

TRENDS IN CELL BIOLOGY | 2009年 / 19卷 / 11期

关键词：

FOCAL ADHESION KINASE; SCANNING FLUORESCENCE MICROSCOPY; ENDOGENOUS STRUCTURAL PROTEINS; VIVO MULTIPHOTON MICROSCOPY; GENE-EXPRESSION SIGNATURE; ORIENTED COLLAGEN GELS; BREAST-CARCINOMA CELLS; IN-VIVO; 2ND-HARMONIC GENERATION; MAMMARY-TUMORS;

D O I：

10.1016/j.tcb.2009.08.009

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Understanding tissue architecture and physical and chemical reciprocity between cells and their microenvironment provides vital insights into key events in cancer metastasis, such as cell migration through three-dimensional (3D) extracellular matrices. Yet, many mechanistic details associated with metastasis remain elusive due to the difficulty of studying cancer cells in relevant 3D microenvironments. Recently, optical imaging has facilitated the direct observation of single cells as they undertake fundamental steps in the metastatic processes. Optical imaging is also providing novel 'optical biomarkers' with diagnostic potential that are linked to cell-motility pathways associated with metastasis, and these can to help guide new approaches to cancer diagnosis and therapy. Here we present recent advances in one subclass of optical imaging of particular promise for cellular imaging - multiphoton microscopy - that can be used to improve the detection of malignant cells as well as advance our understanding of the cell biology of cancer metastasis.

引用

页码：638 / 648

页数：11

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