Statistical Assessment of Variability of Terminal Restriction Fragment Length Polymorphism Analysis Applied to Complex Microbial Communities

被引：16

作者：

Rossi, Pierre ^{[1
]}

Gillet, Francois ^{[2
]}

Rohrbach, Emmanuelle ^{[1
]}

Diaby, Nouhou ^{[1
]}

Holliger, Christof ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, ENAC ISTE Lab Environm Biotechnol, Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, ENAC ISTE Lab Ecosyst, Lausanne, Switzerland

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 22期

关键词：

RIBOSOMAL-RNA GENES; BACTERIAL COMMUNITIES; DIVERSITY; PROFILES; PATTERNS; PRIMERS; BIAS;

D O I：

10.1128/AEM.00135-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The variability of terminal restriction fragment polymorphism analysis applied to complex microbial communities was assessed statistically. Recent technological improvements were implemented in the successive steps of the procedure, resulting in a standardized procedure which provided a high level of reproducibility.

引用

页码：7268 / 7270

页数：3

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