Analysis of changes in the viability and gene expression profiles of human mesenchymal stromal cells over time

被引:25
作者
Lee, Kyoung A. [2 ]
Shim, Wooyoung [2 ,3 ]
Paik, Man Jeong [3 ]
Lee, Soo Cheol [2 ]
Shin, Jin Young [3 ,4 ]
Ahn, Young Hwan [3 ,4 ]
Park, Kiho [5 ]
Kim, Jeong Hyun [2 ]
Choi, Sangdun [2 ]
Lee, Gwang [1 ,2 ,3 ]
机构
[1] Ajou Univ, Sch Med, Inst Med Sci, Suwon 443721, South Korea
[2] Ajou Univ, Dept Mol Sci & Technol, Suwon 443721, South Korea
[3] Ajou Univ, Inst Neuroregenerat & Stem Cell Res, Sch Med, Suwon 443721, South Korea
[4] Ajou Univ, Sch Med, Dept Neurosurg, Suwon 443721, South Korea
[5] Wallace Mem Baptist Hosp, Dept Pediat, Pusan, South Korea
基金
新加坡国家研究基金会;
关键词
cell viability; human mesenchymal stromal cells; microarray; transplantation; MARROW STEM-CELLS; BONE-MARROW; DIFFERENTIATION; TRANSPLANTATION; ANGIOGENESIS; EXTRACTION; EXPANSION; RECOVERY; THERAPY; STROKE;
D O I
10.3109/14653240902974032
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Background aims Because of their ability to differentiate and widespread availability, human mesenchymal stromal cells (hMSC) are often used as a clinical therapeutic tool. However, the factors that determine the quality and viability of hMSC are not well understood. Methods We evaluated the viability of hMSC over time using flow cytometry analysis (FACS) and transmission electron microscopy (TEM) to determine if morphologic changes occurred in hMSC. In addition, we conducted gene expression profiling using an Affymetrix Human Genome U133 Plus 2.0 Array. Results FACS analysis revealed that 83% and 76% of the cells were viable in sterilized phosphate-buffered saline (PBS) after 6 h and 12 h, respectively. TEM data revealed that the total number of cells with healthy chromatins or a few cytosolic vacuoles was significantly reduced over time. We then conducted gene expression profiling using a microarray, which revealed changes in the expression of 2949 functional genes. Specifically, among the total of 50 000 gene probes evaluated, the expression levels of apoptosis and stress-related genes were significantly increased over time. Conclusions The results of this study suggest that the viability of hMSC decreases after disassociation from the culture dish and time is an essential factor when considering hMSC as a potential source for stem cell-based direct transplantation.
引用
收藏
页码:688 / 697
页数:10
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