Sulforaphane Increases Cyclin-Dependent Kinase Inhibitor, p21 Protein in Human Oral Carcinoma Cells and Nude Mouse Animal Model to Induce G2/M Cell Cycle Arrest

被引：0

作者：

Kim, Jun-Hee ^{[1
,2
]}

Kwon, Ki Han ^{[3
]}

Jung, Ji-Youn ^{[4
]}

Han, Hye-Suk ^{[1
,2
]}

Shim, Jung Hyun ^{[1
,2
]}

Oh, SeJun ^{[1
,2
]}

Choi, Kyeong-Hee ^{[1
,2
]}

Choi, Eun-Sun ^{[1
,2
]}

Shin, Ji-Ae ^{[1
,2
]}

Leem, Dae-Ho ^{[5
]}

Soh, Yunjo ^{[2
,6
]}

Cho, Nam-Pyo ^{[1
,2
]}

Cho, Sung-Dae ^{[1
,2
]}

机构：

[1] Chonbuk Natl Univ, Dept Oral Pathol, Sch Dent, Jeonju 561756, South Korea

[2] Chonbuk Natl Univ, Inst Oral Biosci, Brain Korea Project 21, Jeonju 561756, South Korea

[3] Gwangju Univ, Dept Complementary & Alternat Therapy, Coll Management Commerce & Social Welf, Kwangju 503703, South Korea

[4] Kongju Natl Univ, Dept Compan & Lab Anim Sci, Yesan 340702, South Korea

[5] Chonbuk Natl Univ, Dept Oral & Maxillofacial Surg, Sch Dent & Res Clin Med, Jeonju 561756, South Korea

[6] Chonbuk Natl Univ, Dept Dent Pharmacol, Sch Dent, Jeonju 561756, South Korea

来源：

JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION | 2010年 / 46卷 / 01期

关键词：

Sulforaphane; Human Oral Squamous cell carcinoma; G(2)/M arrest; p21; cyclin B; PROSTATE-CANCER CELLS; CASPASE-MEDIATED APOPTOSIS; TRANSCRIPTIONAL REGULATION; GROWTH-INHIBITION; BREAST-CANCER; DNA-DAMAGE; IN-VIVO; EXPRESSION; VEGETABLES; GENE;

D O I：

暂无

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Previously, our group reported that sulforaphane (SFN), a naturally occurring chemopreventive agent from cruciferous vegetables, effectively inhibits the proliferation of KB and YD-10B human oral squamous carcinoma cells by causing apoptosis. In this study, treatment of 20 and 40 mu M of SFN for 12 h caused a cell cycle arrest in the G(2)/M phase. Cell cycle arrest induced by SFN was associated with a significant increase in the p21 protein level and a decrease in cyclin B expression, but there was no change in the cyclin A protein level. In addition, SFN increased the p21 promoter activity significantly. Furthermore, SFN induced p21 protein expression in a nude mouse xenograft model suggesting that SFN is a potent inducer of the p21 protein in human oral squamous carcinoma cells. These findings show that SFN is a promising candidate for molecular-targeting chemotherapy against human oral squamous cell carcinoma.

引用

页码：60 / 67

页数：8

共 44 条

[1] p21WAF1 is required for butyrate-mediated growth inhibition of human colon cancer cells [J].