High internal phase emulsions stabilised by supramolecular cellulose nanocrystals and their application as cell-adhesive macroporous hydrogel monoliths

被引:116
作者
Liu, Sa [1 ]
Jin, Min [1 ]
Chen, Yunhua [1 ]
Gao, Huichang [2 ]
Shi, Xuetao [2 ]
Cheng, Wenhua [1 ]
Ren, Li [1 ]
Wang, Yingjun [2 ]
机构
[1] South China Univ Technol, Sch Mat Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China
[2] South China Univ Technol, Natl Engn Res Ctr Tissue Restorat & Reconstruct, Guangzhou 510006, Guangdong, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
BIOMEDICAL APPLICATIONS;
D O I
10.1039/c7tb00145b
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Nanosized celluloses are attractive building blocks to generate hierarchically advanced materials and have been gradually explored in emulsion applications. Here we report a high internal phase emulsion (HIPE) prepared by using supramolecular cellulose nanocrystals (CNCs) as Pickering stabilisers via one-step emulsification, and interconnected macroporous hybrid hydrogels were produced by utilizing this HIPE as a template. A quadruple hydrogen bonding moiety 2-ureido-4[1H]-pyrimidone (UPy) was firstly grafted onto the surface of cellulose nanocrystals through simple free radical polymerization. The polymer grafting was confirmed by elemental analysis and thermogravimetry. The UPy modified CNCs (CNC-UPy) exhibited superior emulsion stabilising ability compared to the pristine CNCs, and the oil-in-water emulsions with an internal phase volume ratio of 80% showed good long-term stability. The properties of resulting macroporous polyHIPE hydrogels, such as swelling behaviours, porous structures and mechanical strength, were investigated on the dependence of CNC-UPy concentrations. In addition, the macroporous hybrid hydrogel exhibits excellent cytocompatibility and cell adhesion as demonstrated by mouse bone mesenchymal stem cell (mBMSC) culture. With these promising properties, the developed hydrogels demonstrate great potential as active biological scaffolds for tissue engineering.
引用
收藏
页码:2671 / 2678
页数:8
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